Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs
Gil, Olga (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Díaz, Ivan (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Vilaplana i Massaguer, Cristina (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Tapia, Gustavo (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Díaz, Jorge (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Fort, Maria (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Cáceres, Neus (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pinto, Sergio (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Caylà, Joan A.. (Agència de Salut Pública de Barcelona)
Corner, Leigh (University College Dublin. School of Agriculture, Food Science and Veterinary Medicine)
Domingo, Mariano (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Cardona, Pere-Joan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Fecha:	2010
Resumen:	A transthoracic infection involving a low dose of Mycobacterium tuberculosis has been used to establish a new model of infection in minipigs. The 20-week monitoring period showed a marked Th1 response and poor humoral response for the whole infection. A detailed histopathological analysis was performed after slicing the formalin-fixed whole lungs of each animal. All lesions were recorded and classified according to their microscopic aspect, their relationship with the intralobular connective network and their degree of maturity in order to obtain a dissemination ratio (DR) between recent and old lesions. CFU counts and evolution of the DR with time showed that the proposed model correlated with a contained infection, decreasing from week 9 onwards. These findings suggest that the infection induces an initial Th1 response, which is followed by local fibrosis and encapsulation of the granulomas, thereby decreasing the onset of new lesions. Two therapeutic strategies were applied in order to understand how they could influence the model. Thus, chemotherapy with isoniazid alone helped to decrease the total number of lesions, despite the increase in DR after week 9, with similar kinetics to those of the control group, whereas addition of a therapeutic M. tuberculosis fragment-based vaccine after chemotherapy increased the Th1 and humoral responses, as well as the number of lesions, but decreased the DR. By providing a local pulmonary structure similar to that in humans, the mini-pig model highlights new aspects that could be key to a better understanding tuberculosis infection control in humans.
Ayudas:	Ministerio de Ciencia e Innovación BIO2005/07949-CO2-O2 European Commission 2007-2013
Nota:	Altres ajuts: MISA/FIS/PI080785
Nota:	Altres ajuts: I+D+I/FIS/CM06/00123
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; Versió publicada
Materia:	Tuberculosi ; Porcs (Animals de laboratori) ; Mycobacterium tuberculosis
Publicado en:	PloS one, Vol. 5, Issue 4 (April 2010) , p. e10030, ISSN 1932-6203

DOI: 10.1371/journal.pone.0010030
PMID: 20386605

16 p, 1.4 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Artículos > Artículos publicados