Lipid droplet biogenesis induced by stress involves triacylglycerol synthesis that depends on group VIA phospholipase A2
Gubern Burset, Albert (Universitat Autònoma de Barcelona. Institut de Neurociències)
Barceló Torns, Miquel (Universitat Autònoma de Barcelona. Institut de Neurociències)
Casas, Javier (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Biología y Genética Molecular)
Barneda, David (Universitat Autònoma de Barcelona. Institut de Neurociències)
Masgrau Juanola, Roser (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Picatoste Ramón, Fernando (Universitat Autònoma de Barcelona. Institut de Neurociències)
Balsinde, Jesús (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Biología y Genética Molecular)
Balboa María, A. (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Biología y Genética Molecular)
Claro Izaguirre, Enrique (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Date:	2009
Abstract:	This work investigates the metabolic origin of triacylglycerol (TAG) formed during lipid droplet (LD) biogenesis induced by stress. Cytotoxic inhibitors of fatty acid synthase induced TAG synthesis and LD biogenesis in CHO-K1 cells, in the absence of external sources of fatty acids. TAG synthesis was required for LD biogenesis and was sensitive to inhibition and down-regulation of the expression of group VIA phospholipase A2 (iPLA2-VIA). Induction of stress with acidic pH, C2-ceramide, tunicamycin, or deprivation of glucose also stimulated TAG synthesis and LD formation in a manner dependent on iPLA2-VIA. Overexpression of the enzyme enhanced TAG synthesis from endogenous fatty acids and LD occurrence. During stress, LD biogenesis but not TAG synthesis required phosphorylation and activation of group IVA PLA2 (cPLA2α). The results demonstrate that iPLA2-VIA provides fatty acids for TAG synthesis while cPLA2α allows LD biogenesis. LD biogenesis during stress may be a survival strategy, recycling structural phospholipids into energy-generating substrates.
Grants:	Ministerio de Educación y Ciencia SAF2004/01698 Ministerio de Educación y Ciencia SAF2007/60055 Ministerio de Educación y Ciencia BFU2007/67154/BMC
Note:	Altres ajuts: MSPS/PI03/0528
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Acidic pH ; Ceramide ; Cytotoxic ; Down-regulation ; External sources ; Fatty acid synthase ; Lipid droplets ; Over-expression ; Phospholipase A ; Triacylglycerol ; Tunicamycin
Published in:	Journal of biological chemistry, Vol. 284, Num. 9 (2009) , p. 5697-56708, ISSN 1083-351X

DOI: 10.1074/jbc.M806173200
PMID: 19117952

12 p, 853.4 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles