The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress
Galbiati, Massimo (Università degli Studi di Milano. Dipartimento di Scienze Biomolecolari e Biotecnologie)
Matus, José Tomás (Centre de Recerca en Agrigenòmica)
Francia, Priscilla (Università degli Studi di Milano. Dipartimento di Scienze Biomolecolari e Biotecnologie)
Rusconi, Fabio (Fondazione Filarete (Milano, Itàlia))
Cañón, Paola (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Medina, Consuelo (Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)
Conti, Lucio (Università degli Studi di Milano. Dipartimento di Scienze Biomolecolari e Biotecnologie)
Cominelli, Eleonora (Università degli Studi di Milano. Dipartimento di Scienze Biomolecolari e Biotecnologie)
Tonelli, Chiara (Università degli Studi di Milano. Dipartimento di Scienze Biomolecolari e Biotecnologie)
Arce-Johnson, Patricio (Pontificia Universidad Católica de Chile. Departamento de Genética Molecular y Microbiología)

Date:	2011
Abstract:	- Background: Under drought, plants accumulate the signaling hormone abscisic acid (ABA), which induces the rapid closure of stomatal pores to prevent water loss. This event is trigged by a series of signals produced inside guard cells which finally reduce their turgor. Many of these events are tightly regulated at the transcriptional level, including the control exerted by MYB proteins. In a previous study, while identifying the grapevine R2R3 MYB family, two closely related genes, VvMYB30 and VvMYB60 were found with high similarity to AtMYB60, an Arabidopsis guard cell-related drought responsive gene. - Results: Promoter-GUS transcriptional fusion assays showed that expression of VvMYB60 was restricted to stomatal guard cells and was attenuated in response to ABA. Unlike VvMYB30, VvMYB60 was able to complement the loss-of-function atmyb60-1 mutant, indicating that VvMYB60 is the only true ortholog of AtMYB60 in the grape genome. In addition, VvMYB60 was differentially regulated during development of grape organs and in response to ABA and drought-related stress conditions. - Conclusions: These results show that VvMYB60 modulates physiological responses in guard cells, leading to the possibility of engineering stomatal conductance in grapevine, reducing water loss and helping this species to tolerate drought under extreme climatic conditions.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	BMC plant biology, Vol. 11 (Oct. 2011) , art. 142, ISSN 1471-2229

DOI: 10.1186/1471-2229-11-142
PMID: 22018045

15 p, 3.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles