Expression of the maize ZmGF14-6 gene in rice confers tolerance to drought stress while enhancing susceptibility to pathogen infection
Campo, Sonia (Centre de Recerca en Agrigenòmica)
Peris Peris, Cristina (Centre de Recerca en Agrigenòmica)
Montesinos Barreda, Laura (Centre de Recerca en Agrigenòmica)
Peñas Civit, Gisela (Centre de Recerca en Agrigenòmica)
Messeguer, Joaquima (Centre de Recerca en Agrigenòmica)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Data:	2011
Resum:	14-3-3 proteins are found in all eukaryotes where they act as regulators of diverse signalling pathways associated with a wide range of biological processes. In this study the functional characterization of the ZmGF14-6 gene encoding a maize 14-3-3 protein is reported. Gene expression analyses indicated that ZmGF14-6 is up-regulated by fungal infection and salt treatment in maize plants, whereas its expression is down-regulated by drought stress. It is reported that rice plants constitutively expressing ZmGF14-6 displayed enhanced tolerance to drought stress which was accompanied by a stronger induction of drought-associated rice genes. However, rice plants expressing ZmGF14-6 either in a constitutive or under a pathogen-inducible regime showed a higher susceptibility to infection by the fungal pathogens Fusarium verticillioides and Magnaporthe oryzae. Under infection conditions, a lower intensity in the expression of defence-related genes occurred in ZmGF14-6 rice plants. These findings support that ZmGF14-6 positively regulates drought tolerance in transgenic rice while negatively modulating the plant defence response to pathogen infection. Transient expression assays of fluorescently labelled ZmGF14-6 protein in onion epidermal cells revealed a widespread distribution of ZmGF14-6 in the cytoplasm and nucleus. Additionally, colocalization experiments of fluorescently labelled ZmGF14-6 with organelle markers, in combination with cell labelling with the endocytic tracer FM4-64, revealed a subcellular localization of ZmGF14-6 in the early endosomes. Taken together, these results improve our understanding of the role of ZmGF14-6 in stress signalling pathways, while indicating that ZmGF14-6 inversely regulates the plant response to biotic and abiotic stresses.
Ajuts:	Ministerio de Ciencia e Innovación BIO2009-08719 Ministerio de Ciencia e Innovación CSD2007-00036 Ministerio de Ciencia e Innovación BIO2006-05583 Agència de Gestió d'Ajuts Universitaris i de Recerca SGR-09626
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	14-3-3 proteins ; Abiotic stress ; Biotic stress ; Early endosomes ; Fusarium verticillioides ; Drought ; Magnaporthe oryzae ; Oryza sativa ; Zea mays ; ZmPR4 promoter
Publicat a:	Journal of experimental botany, Vol. 63, issue 2 (Jan. 2012) , p. 983-999, ISSN 1460-2431

DOI: 10.1093/jxb/err328
PMID: 22016430

17 p, 1.0 MB

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