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| Home > Articles > Published articles > In Vivo Theranostics at the Peri-Infarct Region in Cerebral Ischemia |
(Universidade de Santiago de Compostela) (Universidade de Santiago de Compostela)| Date: | 2013 |
| Abstract: | The use of theranostics in neurosciences has been rare to date because of the limitations imposed on the free delivery of substances to the brain by the blood-brain barrier. Here we report the development of a theranostic system for the treatment of stroke, a leading cause of death and disability in developed countries. We first performed a series of proteomic, immunoblotting and immunohistological studies to characterize the expression of molecular biomarkers for the so-called peri-infarct tissue, a key region of the brain for stroke treatment. We confirmed that the HSP72 protein is a suitable biomarker for the peri-infarct region, as it is selectively expressed by at-risk tissue for up to 7 days following cerebral ischemia. We also describe the development of anti-HSP72 vectorized stealth immunoliposomes containing imaging probes to make them traceable by conventional imaging techniques (fluorescence and MRI) that were used to encapsulate a therapeutic agent (citicoline) for the treatment of cerebral ischemia. We tested the molecular recognition capabilities of these nano-platforms in vitro together with their diagnostic and therapeutic properties in vivo, in an animal model of cerebral ischemia. Using MRI, we found that 80% of vectorized liposomes were located on the periphery of the ischemic lesion, and animals treated with citicoline encapsulated on these liposomes presented lesion volumes up to 30% smaller than animals treated with free (non-encapsulated) drugs. Our results show the potential of nanotechnology for the development of effective tools for the treatment of neurological diseases. |
| Grants: | Instituto de Salud Carlos III PI11-02161 Instituto de Salud Carlos III CP09-00074 Ministerio de Economía y Competitividad SAF2008-02190 Ministerio de Economía y Competitividad SAF2011-30517 Ministerio de Economía y Competitividad RETICS-INVICTUS/R012-0014 |
| Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.  |
| Language: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Subject: | Cerebral ischemia ; Peri-infarct region ; MRI ; Theranostics ; Drug delivery |
| Published in: | Theranostics, Vol. 4 (december 2013) , p. 90-105, ISSN 1838-7640 |
