Paracrine brassinosteroid signaling at the stem cell niche controls cellular regeneration
Lozano Elena, Fidel (Centre de Recerca en Agrigenòmica)
Planas Riverola, Ainoa (Centre de Recerca en Agrigenòmica)
Vilarrasa Blasi, Josep (Centre de Recerca en Agrigenòmica)
Schwab, Rebecca (Cold Spring Harbor Laboratory)
Caño Delgado, Ana I. (Centre de Recerca en Agrigenòmica)

Date:	2018
Abstract:	Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) act as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of the QC have a very low mitotic activity, plant hormones such as brassinosteroids (BRs) can promote QC divisions. Here, we used a tissue-specific strategy to investigate the spatial signaling requirements of BR-mediated QC divisions. We generated stem cell niche-specific receptor knockout lines by placing an artificial microRNA against BRI1 (BRASSINOSTEROID INSENSITIVE 1) under the control of the QC-specific promoter WOX5. Additionally, QC-specific knock-in lines for BRI1 and its downstream transcription factor BES1 (BRI1-EMS-SUPPRESOR1) were also created using the WOX5 promoter. By analyzing the roots of these lines, we show that BES1-mediated signaling cell-autonomously promotes QC divisions, that BRI1 is essential for sensing nearby inputs and triggering QC divisions and that DNA damage promotes BR-dependent paracrine signaling in the stem cell niche as a prerequisite to stem cell replenishment.
Grants:	European Commission 683163 Ministerio de Economía y Competitividad BIO2013-43873 Ministerio de Economía y Competitividad BIO2016-78955 Ministerio de Economía y Competitividad SEV-2015-0533
Note:	Altres ajuts: EMBO/ALTF864-2005
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Brassinosteroid ; Quiescent center ; Cell division ; Stem cell ; DNA damage ; Paracrine
Published in:	Journal of cell science, Vol. 131, Issue 2 (January 2018) , p. 1-11, ISSN 0021-9533

DOI: 10.1242/jcs.204065
PMID: 29242230

21 p, 16.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles