The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
Millares, Laura (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Bermudo, Guadalupe (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pérez-Brocal, Vicente (Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO))
Domingo, Christian (Universitat Autònoma de Barcelona. Departament de Medicina)
Garcia-Nuñez, Marian (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pomares, Xavier (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Moya, Andrés (Valencia, Spain)
Monsó, Eduard (Universitat Autònoma de Barcelona. Departament de Medicina)

Date:	2017
Abstract:	The bronchial microbiome in chronic lung diseases presents an abnormal pattern, but its microbial composition and regional differences in severe asthma have not been sufficiently addressed. The aim of the study was to describe the bacterial community in bronchial mucosa and secretions of patients with severe chronic asthma chronically treated with corticosteroids in addition to usual care according to Global Initiative for Asthma. Bacterial community composition was obtained by 16S rRNA gene amplification and sequencing, and functional capabilities through PICRUSt. Thirteen patients with severe asthma were included and provided 11 bronchial biopsies (BB) and 12 bronchial aspirates (BA) suitable for sequence analyses. Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria showed relative abundances (RAs) over 5% in BB, a cutoff that was reached by Streptococcus and Prevotella at genus level. Legionella genus attained a median RA of 2. 7 (interquartile range 1. 1-4. 7) in BB samples. In BA a higher RA of Fusobacteria was found, when compared with BB [8. 7 (5. 9-11. 4) vs 4. 2 (0. 8-7. 5), p = 0. 037], while the RA of Proteobacteria was lower in BA [4. 3 (3. 7-6. 5) vs 17. 1 (11. 2-33. 4), p = 0. 005]. RA of the Legionella genus was also significantly lower in BA [0. 004 (0. 001-0. 02) vs. 2. 7 (1. 1-4. 7), p = 0. 005]. Beta-diversity analysis confirmed the differences between the microbial communities in BA and BB (R 2 = 0. 20, p = 0. 001, Adonis test), and functional analysis revealed also statistically significant differences between both types of sample on Metabolism, Cellular processes, Human diseases, Organismal systems and Genetic information processing pathways. The microbiota in the bronchial mucosa of severe asthma has a specific pattern that is not accurately represented in bronchial secretions, which must be considered a different niche of bacteria growth. The online version of this article (doi:10. 1186/s12866-017-0933-6) contains supplementary material, which is available to authorized users.
Note:	Altres ajuts: This work was supported by Fundació Catalana de Neumología (FUCAP) and Centro de Investigación Biomédica en Red de Enfermedades Respiratorias(CIBERES). CIBERES is an initiative of the Instituto de Salud Carlos III.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Microbiome ; Asthma ; Biopsy ; Bronchial aspirate
Published in:	BMC Microbiology, Vol. 17 (january 2017) , ISSN 1471-2180

DOI: 10.1186/s12866-017-0933-6
PMID: 28103814

11 p, 932.7 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Parc Taulí Research and Innovation Institute (I3PT
Articles > Research articles
Articles > Published articles