The effects of cobalt protoporphyrin IX and tricarbonyldichlororuthenium (II) dimer treatments and its interaction with nitric oxide in the locus coeruleus of mice with peripheral inflammation
Moreno, Patricia (Institut d'Investigació Biomèdica Sant Pau)
Cazuza, R. A. (Department of Psychology. University of São Paulo)
Gomes-Mendes, J. (Department of Psychology. University of São Paulo)
Díaz, A. F. (Universitat Autònoma de Barcelona. Institut de Neurociències)
Polo, S. (Universitat Autònoma de Barcelona. Institut de Neurociències)
Leánez, Sergi (Universitat Autònoma de Barcelona. Institut de Neurociències)
Leite-Panissi, C. R. A. (Department of Psychology. University of São Paulo)
Pol, Olga. (Universitat Autònoma de Barcelona. Institut de Neurociències)
Universitat Autònoma de Barcelona

Date:	2019
Abstract:	Heme oxygenase 1 (HO-1) and carbon monoxide were shown to normalize oxidative stress and inflammatory reactions induced by neuropathic pain in the central nervous system, but their effects in the locus coeruleus (LC) of animals with peripheral inflammation and their interaction with nitric oxide are unknown. In wild-type (WT) and knockout mice for neuronal (NOS1-KO) or inducible (NOS2-KO) nitric oxide synthases with inflammatory pain induced by complete Freund's adjuvant (CFA), we assessed: 1) antinociceptive actions of cobalt protoporphyrin IX (CoPP), an HO-1 inducer; 2) effects of CoPP and tricarbonyldichlororuthenium(II)dimer (CORM-2), a carbon monoxide-liberating compound, on the expression of HO-1, NOS1, NOS2, CD11b/c, GFAP,and mitogen-activated protein kinases (MAPK)in the LC. CoPP reduced inflammatory pain in different time-dependent manners in WT and KO mice. Peripheral inflammation activated astroglia in the LC of all genotypes and increased the levels of NOS1 and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK 1/2) in WT mice. CoPP and CORM-2 enhanced HO-1 and inhibited astroglial activationin all genotypes. Both treatments blocked NOS1 overexpression,and CoPP normalized ERK 1/2 activation. This study reveals an interaction between HO-1 and NOS1/NOS2 during peripheral inflammation andshows that CoPP and CORM-2 improved HO-1 expression and modulated the inflammatory and/or plasticity changes caused by peripheral inflammation in the LC.
Grants:	Instituto de Salud Carlos III PS0900968 Instituto de Salud Carlos III PI1400927
Note:	Funding: This work was supported by Ministerio de Economía y Competitividad, Instituto de Salud Carlos III, Madrid, Spain and FondoEuropeo de Desarrollo Regional (FEDER), Unión Europea [Grants: PS0900968 and PI1400927], and CAPES/PROEX, CNPq,Brasil [401472/2014-0].
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Analgesia ; Carbon monoxide ; Heme oxygenase 1 ; Inflammatory pain ; Locus coeruleus ; Nitric oxide
Published in:	International journal of molecular sciences, Vol. 20, Issue 9 (January 2019) , p. 2211, ISSN 1422-0067

DOI: 10.3390/ijms20092211
PMID: 31060340

14 p, 1.9 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles