ZmXTH1, a new xyloglucan endotransglucosylase/hydrolase in maize, affects cell wall structure and composition in Arabidopsis thaliana
Genovesi, Valeria (Institut de Recerca i Tecnologia Agroalimentàries)
Fornalé, Silvia (Institut de Recerca i Tecnologia Agroalimentàries)
Fry, Stephen C. (University of Edinburgh. Institute of Molecular Plant Sciences)
Ruel, Katia (Centre National de la Recherche Scientifique (França))
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Encina, Antonio (Universidad de León. Área de Fisiología Vegetal)
Sonbol, Fathi-Mohamed (Institut de Recerca i Tecnologia Agroalimentàries)
Bosch Argilagós, Josep (Institut de Recerca i Tecnologia Agroalimentàries)
Puigdomènech, Pere, 1948- (Institut de Recerca i Tecnologia Agroalimentàries)
Rigau, Joan (Institut de Recerca i Tecnologia Agroalimentàries)
Caparros Ruíz, David (Institut de Recerca i Tecnologia Agroalimentàries)

Date:	2008
Abstract:	Xyloglucan endotransglucosylase/hydrolases (XTHs; EC 2. 4. 1. 207 and/or EC 3. 2. 1. 151) are enzymes involved in the modification of cell wall structure by cleaving and, often, also re-joining xyloglucan molecules in primary plant cell walls. Using a pool of antibodies raised against an enriched cell wall protein fraction, a new XTH cDNA in maize, ZmXTH1, has been isolated from a cDNA expression library obtained from the elongation zone of the maize root. The predicted protein has a putative N-terminal signal peptide and possesses the typical domains of this enzyme family, such as a catalytic domain that is homologous to that of Bacillus macerans β-glucanase, a putative N-glycosylation motif, and four cysteine residues in the central and C terminal regions of the ZmXTH1 protein. Phylogenetic analysis of ZmXTH1 reveals that it belongs to subgroup 4, so far only reported from Poaceae monocot species. ZmXTH1 has been expressed in Pichia pastoris (a methylotrophic yeast) and the recombinant enzyme showed xyloglucan endotransglucosylase but not xyloglucan endohydrolase activity, representing the first enzyme belonging to subgroup 4 characterized in maize so far. Expression data indicate that ZmXTH1 is expressed in elongating tissues, modulated by culture conditions, and induced by gibberellins. Transient expression assays in onion cells reveal that ZmXTH1 is directed to the cell wall, although weakly bound. Finally, Arabidopsis thaliana plants expressing ZmXTH1 show slightly increased xyloglucan endohydrolase activity and alterations in the cell wall structure and composition.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2003/FI00090 Ministerio de Ciencia y Tecnología BIO2001-1140 Agència de Gestió d'Ajuts Universitaris i de Recerca 2005/BE00104 Agència de Gestió d'Ajuts Universitaris i de Recerca 2006/BE00668 Agència de Gestió d'Ajuts Universitaris i de Recerca 2003/PIV-A-00033
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cell elongation ; Cell wall ; Plant transformation ; XEH ; XET ; XTH ; Zea mays
Published in:	Journal of experimental botany, Vol. 59, Issue 4 (March 2008) , p. 875-889, ISSN 1460-2431

DOI: 10.1093/jxb/ern013

15 p, 1.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles