Single-cell telomere-length quantification couples telomere length to meristem activity and stem cell development in Arabidopsis
González-García, Mary Paz (Centre de Recerca en Agrigenòmica)
Pavelescu, Irina (Centre de Recerca en Agrigenòmica)
Canela, Andrés (Centro Nacional de Investigaciones Oncológicas)
Sevillano, Xavier (Universitat Ramon Llull. Grup de Recerca en Tecnologies Mèdia)
Leehy, Katherine A. (Texas A&M University. Department of Biochemistry and Biophysics)
Nelson, Andrew D. L. (Texas A&M University. Department of Biochemistry and Biophysics)
Ibañes, Marta (Universitat de Barcelona. Departament d'Estructura i Constituents de la Matèria)
Shippen, Dorothy E. (Texas A&M University. Department of Biochemistry and Biophysics)
Blasco, María A. (Centro Nacional de Investigaciones Oncológicas)
Caño Delgado, Ana I. (Centre de Recerca en Agrigenòmica)

Date:	2015
Abstract:	Telomeres are specialized nucleoprotein caps that protect chromosome ends assuring cell division. Single-cell telomere quantification in animals established a critical role for telomerase in stem cells, yet, in plants, telomere-length quantification has been reported only at the organ level. Here, a quantitative analysis of telomere length of single cells in Arabidopsis root apex uncovered a heterogeneous telomere-length distribution of different cell lineages showing the longest telomeres at the stem cells. The defects in meristem and stem cell renewal observed in tert mutants demonstrate that telomere lengthening by TERT sets a replicative limit in the root meristem. Conversely, the long telomeres of the columella cells and the premature stem cell differentiation plt1,2 mutants suggest that differentiation can prevent telomere erosion. Overall, our results indicate that telomere dynamics are coupled to meristem activity and continuous growth, disclosing a critical association between telomere length, stem cell function, and the extended lifespan of plants.
Grants:	European Commission 259749 European Commission 232854 European Commission 20088 Ministerio de Economía y Competitividad BIO2013-43873 Ministerio de Ciencia e Innovación FIS2012-37655-C02-02 Ministerio de Economía y Competitividad BIO2010-16673 Ministerio de Economía y Competitividad SAF2008-05384 Ministerio de Economía y Competitividad CSD2007-00017 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-878
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Arabidopsis ; Arabidopsis Proteins ; Cell Compartmentation ; Cell Differentiation ; Cell Division ; In Situ Hybridization ; Fluorescence ; Meristem ; Mutation ; Single-Cell Analysis ; Stem Cell Niche ; Stem Cells ; Telomerase ; Telomere
Published in:	Cell reports, Vol. 11, issue 6 (May 2015) , p. 977-989, ISSN 2211-1247

DOI: 10.1016/j.celrep.2015.04.013
PMID: 25937286

14 p, 5.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles