Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water
Hassan, Abdel-Raim H. A. (Institut Català de Nanociència i Nanotecnologia)
Bergua Canudo, José Francisco (Institut Català de Nanociència i Nanotecnologia)
Morales-Narváez, Eden (Biophotonic Nanosensors Laboratory)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2019
Abstract:	Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for determination of E. coli O157:H7 in beef and river water. FLFIA showed a high sensitivity rate thousand-fold better than the conventional lateral flow (LF). In inoculated minced beef and river water samples, the limits of detection were 178 and 133 CFU g or mL, respectively. Besides, it presented a high selectivity in the presence of other possible interfering bacteria. The single antibody approach reduced the assay cost to 60% less than the conventional LF. Alongside, the results could be read by portable LF readers or smartphones. These advantages offer FLFIA as a promising technology for pathogen detection in food and water.
Grants:	Ministerio de Economía y Competitividad SEV-2017-0706
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Graphene oxide ; Fluorescent lateral flow ; E. coli O157:H7 ; Food safety ; Minced beef ; Water quality
Published in:	Food chemistry, Vol. 297 (November 2019) , art. 124965, ISSN 1873-7072

DOI: 10.1016/j.foodchem.2019.124965

Postprint 34 p, 909.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles