Mimicry technology : a versatile tool for small RNA suppression
Villar Martin, Luis Manuel (Centre de Recerca en Agrigenòmica)
Rubio-Somoza, Ignacio (Centre de Recerca en Agrigenòmica)

Imprint:	New York : Humana Press, 2019
Description:	7 pàg.
Abstract:	A decade ago the discovery of the target mimicry regulatory process on the activity of a mature microRNA (miRNA) enabled for the first time the customized attenuation of miRNA activity in plants. That powerful technology was named MIMIC and was based on engineering the IPS1 long noncoding transcript to become complementary to the miRNA under study. In order to avoid IPS1 degradation, the predicted miRNA-mediated cleavage site was interrupted by three additional nucleotides giving rise to the so-called MIMIC decoy. Since then, MIMIC technology has been used in several plant species and in basic and translational research. We here provide a detailed guide to produce custom-designed MIMIC decoys to facilitate the study of sRNA functions in plants.
Grants:	Ministerio de Economía y Competitividad BES-2016-076986 Ministerio de Economía y Competitividad RYC-2015-19154 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad BFU-2014-58361-JIN
Rights:	Tots els drets reservats.
Language:	Anglès
Series:	Methods in molecular biology ; 1932
Document:	Capítol de llibre ; Versió acceptada per publicar
Subject:	Target mimicry ; Mirna ; Small RNA attenuation ; MIMIC
Published in:	Plant MicroRNAs, 2019, p. 27-38, ISBN 978-1-4939-9041-2

DOI: 10.1007/978-1-4939-9042-9_18

Post-print 8 p, 490.5 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Books and collections > Book chapters