Legionella SBT applied directly to respiratory samples as a rapid molecular epidemiological tool
Quero, Sara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Párraga-Niño, Noemí (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Sabriá-Leal, M. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Barrabeig, Irene (Agència de Salut Pública de Catalunya)
Sala, M. R. (Agència de Salut Pública de Catalunya)
Jané i Checa, Mireia (Agència de Salut Pública de Catalunya)
Mateu, Lourdes (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Sopena, Nieves (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Pedro-Botet, M. L. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Garcia-Nuñez, Marian (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Universitat Autònoma de Barcelona

Date:	2019
Abstract:	Legionnaires' disease (LD) is an atypical pneumonia caused by the inhalation of Legionella. The methods used for the diagnosis of LD are direct culture of respiratory samples and urinary antigen detection. However, the sensitivity of culture is low, and the urinary antigen test is specific only for L. pneumophila sg1. Moreover, as no isolates are obtained, epidemiological studies cannot be performed. The implementation of Nested-sequence-based typing (Nested-SBT) makes it possible to carry out epidemiological studies while also confirming LD, especially in cases caused by non-sg 1. Sixty-two respiratory samples from patients with Legionella clinically confirmed by positive urinary antigen tests were cultured and tested by Nested-SBT, following the European Study Group for Legionella Infections (ESGLI) protocol. Only 2/62 (3. 2%) respiratory samples were culture-positive. Amplification and sequencing of Nested-SBT genes were successfully performed in 57/62 samples (91. 9%). The seven target genes were characterised in 39/57 (68. 4%) respiratory samples, and the complete sequence type (ST) was obtained. The mip gene was the most frequently amplified and sequenced. Nested-SBT is a useful method for epidemiological studies in culture-negative samples, achieving a 28. 7-fold improvement over the results of culture studies and reducing the time needed to obtain molecular epidemiological results.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Alleles ; Humans ; Legionella ; Legionella pneumophila ; Legionnaires' Disease ; Molecular Diagnostic Techniques ; Molecular Epidemiology ; Sequence Analysis ; Methods
Published in:	Scientific reports, Vol. 9 Núm. 1 (january 2019) , p. 623, ISSN 2045-2322

DOI: 10.1038/s41598-018-36924-w
PMID: 30679570

6 p, 1.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Parc Taulí Research and Innovation Institute (I3PT
Articles > Research articles
Articles > Published articles