Blocking transmission of Middle East respiratory syndrome coronavirus (MERS-CoV) in llamas by vaccination with a recombinant spike protein
Rodon, Jordi (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Okba, Nisreen M. A. (Erasmus Medical Centre. Department of Viroscience)
Te, Nigeer (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
van Dieren, Brenda (Utrecht University. Department of Infectious Diseases & Immunology)
Bosch, Berend-Jan (Utrecht University. Department of Infectious Diseases & Immunology)
Bensaid, Albert (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Segalés Coma, Joaquim (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Haagmans, Bart L. (Erasmus Medical Centre. Department of Viroscience)
Vergara-Alert, Júlia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Date:	2019
Abstract:	The ongoing Middle East respiratory syndrome coronavirus (MERS-CoV) outbreaks pose a worldwide public health threat. Blocking MERS-CoV zoonotic transmission from dromedary camels, the animal reservoir, could potentially reduce the number of primary human cases. Here we report MERS-CoV transmission from experimentally infected llamas to naïve animals. Directly inoculated llamas shed virus for at least 6 days and could infect all in-contact naïve animals 4-5 days after exposure. With the aim to block virus transmission, we examined the efficacy of a recombinant spike S1-protein vaccine. In contrast to naïve animals, in-contact vaccinated llamas did not shed infectious virus upon exposure to directly inoculated llamas, consistent with the induction of strong virus neutralizing antibody responses. Our data provide further evidence that vaccination of the reservoir host may impede MERS-CoV zoonotic transmission to humans.
Grants:	European Commission INFRA-2016-1 N°731014
Note:	Altres ajuts: IMI/115760
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Animal model ; Llama ; Middle East respiratory syndrome coronavirus ; MERS-CoV ; S1-protein-based vaccine ; Virus transmission
Published in:	Emerging microbes & infections, Vol. 8 (november 2019) , p. 1593-1603, ISSN 2222-1751

DOI: 10.1080/22221751.2019.1685912
PMID: 31711379

11 p, 2.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles