Antimicrobial resistance profile and prevalence of extended-spectrum beta-lactamases (ESBL), AmpC beta-lactamases and colistin resistance (mcr) genes in Escherichia coli from swine between 1999 and 2018
Aguirre Molins, Laia (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Vidal, Anna (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Seminati, Chiara (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Tello, Montse (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Redondo, Noelia (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Darwich Soliva, Laila (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Martín Castillo, Margarita (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Data:	2020
Resum:	The frequent usage of antibiotics in livestock has led to the spread of resistant bacteria within animals and their products, with a global warning in public health and veterinarians to monitor such resistances. This study aimed to determine antibiotic resistance patterns and genes in pig farms from Spain during the last twenty years. Susceptibility to six antibiotics commonly used in pig production was tested by qualitative (disk diffusion) and quantitative (minimum inhibitory concentration, MIC) methods in 200 strains of Escherichia coli which had been isolated between 1999 and 2018 from clinical cases of diarrhoea in neonatal and post-weaned piglets. Results showed resistance around 100% for amoxicillin and tetracycline since 1999, and a progressive increase in ceftiofur resistance throughout the studied period. For colistin, it was detected a resistance peak (17. 5% of the strains) in the 2011-2014 period. Concerning gentamicin, 11 of 30 strains with intermediate susceptibility by the disk diffusion method were resistant by MIC. Besides, the most frequent antimicrobial resistance genes were the extended-spectrum beta-lactamase (ESBL) bla (13. 5% of strains, being CTX-M-14, CTX-M-1 and CTX-M-32 the most prevalent genomes, followed by CTX-M-27, CTX-M-9 and CTX-M-3), AmpC-type beta-lactamase (AmpC) bla (3%) and colistin resistance genes mcr -4 (13%), mcr -1 (7%) and in less proportion mcr -5 (3%). Interestingly, these mcr genes were already detected in strains isolated in 2000, more than a decade before their first description. However, poor concordance between the genotypic mcr profile and the phenotypical testing by MIC was found in this study. These results indicate that although being a current concern, resistance genes and therefore antimicrobial resistant phenotypes were already present in pig farms at the beginning of the century.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Antimicrobial resistance ; Escherichia coli ; Pig ; Colistin-mcr genes ; ESBL
Publicat a:	Porcine health management, Vol. 6 (april 2020) , ISSN 2055-5660

DOI: 10.1186/s40813-020-00146-2
PMID: 32266079

6 p, 670.0 KB

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