Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria
Molina-Moya, Barbara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Abdurrahman, Saddiq T. (National TB, Buruli Ulcer and Leprosy Control Programme, Abuja, Nigeria)
Madukaji, Laura I (Bingham University, Nasarawa State, Nigeria)
Gomgnimbou, Michel Kiréopori (Centre Muraz, Bobo-Dioulasso, Burkina Faso)
Spinasse, Lizania (Université Paris Sud. Institut for Integrative Biology of the Cell)
Gomes Fernandes, Meissiner (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Gomes, Harrison Magdinier (Université Paris Sud. Institut for Integrative Biology of the Cell)
Kacimi, Sarah (Université Paris Sud. Institut for Integrative Biology of the Cell)
Dacombe, Russell (Liverpool School of Tropical Medicine, Liverpool, UK)
Bimba, John (Bingham University. Zankli Research Centre and Department of Community Medicine)
Lawson, Lovett (Bingham University (Nigèria))
Sola, Christophe (Université Paris Sud. Institut for Integrative Biology of the Cell)
Cuevas, Luis E. (Liverpool School of Tropical Medicine)
Domínguez, José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Date:	2018
Abstract:	Nigeria ranks fourth among the high tuberculosis (TB) burden countries. This study describes the prevalence of drug resistance and the genetic diversity of Mycobacterium tuberculosis in Abuja's Federal Capital Territory. Two hundred and seventy-eight consecutive sputum samples were collected from adults with presumptive TB during 2013-2014. DNA was extracted from Löwenstein-Jensen cultures and analyzed for the identification of nontuberculous mycobacteria species, detection of drug resistance with line probe assays, and high-throughput spacer oligonucleotide typing (spoligotyping) using microbead-based hybridization. Two hundred and two cultures were positive for M. tuberculosis complex, 24 negative, 38 contaminated, and 15 positive for nontuberculous mycobacteria. Five (2. 5%) M. tuberculosis complex isolates were resistant to rifampicin (RIF) and isoniazid (multidrug resistant), nine (4. 5%) to RIF alone, and 15 (7. 4%) to isoniazid alone; two RIF-resistant isolates were also resistant to fluoroquinolones and ethambutol, and one multidrug resistant isolate was also resistant to ethambutol. Among the 180 isolates with spoligotyping results, 164 (91. 1%) were classified as lineage 4 (Euro-American), 13 (7. 2%) as lineage 5 (West African 1), two (1. 1%) as lineage 2 (East Asia), and one (0. 6%) as lineage 6 (West African 2). One hundred and fifty-six (86. 7%) isolates were grouped in 17 clusters (2-108 isolates/cluster), of which 108 (60. 0%) were grouped as L4. 6. 2/Cameroon (spoligotype international type 61). The description of drug resistance prevalence and genetic diversity of M. tuberculosis in this study may be useful for improving TB control in Nigeria.
Grants:	Instituto de Salud Carlos III PI13-01546 Instituto de Salud Carlos III PI16-01912
Note:	Altres ajuts: This study was funded by a Strategic Award grant from the European and Developing Countries Clinical Trials Partnership (grant SP.2011.41304.021) and its cofounders, the Medical Research Council UK, and Instituto de Salud Carlos III (ISCIII), Spain. MKG was a Postdoctorate fellow from Centre Muraz, Bobo-Dioulasso, Burkina Faso. LS was a Postdoctorate fellow from UFRJ, Rio de Janeiro, Brazil, working in Sola's Orsay team. MG-F was a PhD student funded by CAPES Foundation, Ministry of Education of Brazil, Brasília, Brazil. JD was funded by the "Miguel Servet" program of ISCIII (Spain).The funders were not involved in any of the stages from study design to submission of the manuscript for publication. We are grateful to all TB program coordinators for the collection of samples in Nigeria.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Tuberculosis ; Isoniazid ; Rifampicin ; Line probe assay ; Microbeads ; Spoligotyping
Published in:	Infection and drug resistance, Vol. 11 (october 2018) , p. 1617-1625, ISSN 1178-6973

DOI: 10.2147/IDR.S166986
PMID: 30319278

9 p, 193.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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