Precipitated sdLDL : An easy method to estimate LDL particle size
Fernández Cidón, Bárbara (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Candás-Estébanez, Beatriz (Hospital Universitari de Bellvitge)
Ribalta, Josep (Institut d'Investigació Sanitària Pere Virgili)
Rock, Edmond (French National Institute for Agricultural Research)
Guardiola Guionnet, Montserrat (Institut d'Investigació Sanitària Pere Virgili)
Amigó, Núria (Institut d'Investigació Sanitària Pere Virgili)
Padró-Miquel, Ariadna (Hospital Universitari de Bellvitge)
Alía Ramos, Pedro (Hospital Universitari de Bellvitge)
Pintó, Xavier (Hospital Universitari de Bellvitge)

Date:	2020
Abstract:	Background. LDL-C lowering is the main measure in cardiovascular disease prevention but a residual risk of ischemic events still remains. Alterations of lipoproteins, specially, increase in small dense LDL (sdLDL) particles are related to this risk. Objective. To investigate the potential use of sdLDL cholesterol concentration (sdLDL-C) isolated by an easy precipitation method and to assess the impact of a set of clinical and biochemical variables determined by NMR on sdLDL concentration. Methods. sdLDL-C and NMR lipid profile were performed in 85 men samples. Association among them was evaluated using Pearson coefficients (rxy). A multivariate regression was performed to identify the influence of NMR variables on sdLDL-C. Results. A strong association between sdLDL-C and LDLLDL-P (rxy = 0. 687) and with LDL-Z (rxy = −0. 603) was found. The multivariate regression explained a 56. 8% in sdLDL-C variation (P = 8. 77. 10-12). BMI, ApoB, triglycerides, FFA, and LDL-Z showed a significant contribution. The most important ones were ApoB and LDL-Z; a 1nm increase (LDL-Z) leads to decrease 126 nmol/L in sdLDL-C. Conclusion. The association between sdLDL-C, LDL-Z, and LDL-P is clear. From a large number of variables, especially LDL-Z and apoB influence on sdLDL-C. Results show that the smaller the LDL size, the higher their cholesterol concentration. Therefore, sdLDL-C determination by using this easy method would be useful to risk stratification and to uncover cardiovascular residual risk.
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Note:	Altres ajuts: This study was funded by Hospital Universitari de Bellvitge, part of the Institut Català de la Salut (ICS) and Department of Biochemistry, Molecular Biology and Biomedicine, Autonomous University of Barcelona (UAB), Barcelona, Spain
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Atherosclerosis ; Lipoprotein precipitation ; Nuclear magnetic resonance lipid profile ; Residual cardiovascular risk ; Small dense LDL
Published in:	Journal of Clinical Laboratory Analysis, Vol. 34, Issue 7 (July 2020) , art. e23282, ISSN 1098-2825

DOI: 10.1002/jcla.23282
PMID: 32198796

9 p, 438.8 KB

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