Leptospira Detection in Cats in Spain by Serology and Molecular Techniques
Murillo Picco, Andrea (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Cuenca Valera, Rafaela (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Serrano Ferron, Emmanuel (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Goris, Marga (University of Amsterdam. OIE and National Collaborating Centre for Reference and Research on Leptospirosis (NRL))
Ahmed, Ahmed (University of Amsterdam. OIE and National Collaborating Centre for Reference and Research on Leptospirosis (NRL))
Cervantes, Salvador (Clínica Felina Barcelona)
Caparrós, Cristina (Clínica Felina Barcelona)
Vieitez, Verónica (Universidad de Extremadura. Facultad de Veterinaria)
Ladina, Andrea (Universidad de Extremadura. Facultad de Veterinaria)
Pastor Milán, Josep (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2020
Abstract:	Leptospirosis is the most neglected widespread zoonosis worldwide. In Spain, leptospirosis reports in people and animals have increased lately. Cats can become infected with Leptospira, as well as be chronic carriers. The aim of this study was to determine serological antibody prevalence against Leptospira sp. , blood DNA, and shedding of DNA from pathogenic Leptospira species in the urine of cats in Spain. Microagglutination tests (MAT) and blood and urine TaqMan real-time polymerase chain reaction (PCR) were performed. Leptospira antibodies were detected in 10/244 cats; with 4. 1% positive results (95% confidence interval (CI): 2. 1-7. 18%). Titers ranged from 1:20 to 1:320 (serovars Ballum; Bataviae; Bratislava; Cynopteri; Grippotyphosa Mandemakers; Grippotyphosa Moskva; Pomona; and Proechimys). The most common serovar was Cynopteri. Blood samples from 1/89 cats amplified for Leptospira DNA (1. 12%; 95% CI: 0. 05-5. 41%). Urine samples from 4/232 cats amplified for Leptospira DNA (1. 72%; 95% CI: 0. 55-4. 10%). In conclusion free-roaming cats in Spain can shed pathogenic Leptospira DNA in their urine and may be a source of human infection. Serovars not previously described in cats in Spain were detected; suggesting the presence of at least 4 different species of pathogenic leptospires in the country (L. borgpetersenii; L. interrogans; L. kirschneri; and.
Abstract:	L. noguchii).
Grants:	Ministerio de Economía y Competitividad RYC-2016-21120
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Leptospirosi ; Gats -- Malalties
Published in:	International journal of environmental research and public health, Vol. 17 (Febrer 2020) , p. 1600, ISSN 1660-4601

DOI: 10.3390/ijerph17051600
PMID: 32121670

11 p, 337.8 KB

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