Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year
Arrieta-Villegas, Claudia (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Martín, Maite (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Verdés Martinez, Judit (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Moll Sánchez, Xavier (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Espada, Yvonne (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Singh, Mahavir (LIONEX Diagnostics and Therapeutics GmbH)
Villarreal Ramos, Bernardo (Animal Health and Veterinary Laboratories Agency. TB Research Group)
Domingo, Mariano (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Pérez de Val, Bernat (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Vidal Barba, Enric (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Fecha:	2020
Resumen:	Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4−CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae.
Nota:	Altres ajuts: INIA/FEDER/RTA2015-00043-C02-01
Nota:	Altres ajuts: INIA-FPI/CPD2016-0109
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Vacunació d'animals ; Cabres ; BCG ; Revaccination ; Duration of immunity; goat ; Tuberculosis ; Vaccine ; Diagnosis ; Interferon gamma ; Antigen-specific memory T-cells
Publicado en:	Vaccines (Basel), Vol. 8 Núm. 4 (2020) , p. 751, ISSN 2076-393X

DOI: 10.3390/vaccines8040751
PMID: 33322064

16 p, 20.5 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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