Size-dependent pulmonary impact of thin graphene oxide sheets in mice : toward safe-by-design
Rodrigues, Artur Filipe (University of Manchester. Nanomedicine Lab)
Newman, Leon (University of Manchester. Nanomedicine Lab)
Jasim, Dhifaf A (University of Manchester. Nanomedicine Lab)
Mukherjee, Sourav P. (Karolinska Institutet (Estocolm, Suècia). Nanosafety & Nanomedicine Laboratory)
Wang, Jun (Stockholm University. Department of Biochemistry and Biophysics)
Vacchi, Isabella A. (Centre National de la Recherche Scientifique (França))
Ménard-Moyon, Cécilia (Centre National de la Recherche Scientifique (França))
Bianco, Alberto (Centre National de la Recherche Scientifique (França))
Fadeel, Bengt (Karolinska Institutet (Estocolm, Suècia). Nanosafety & Nanomedicine Laboratory)
Kostarelos, Kostas (Institut Català de Nanociència i Nanotecnologia)
Bussy, Cyrill (Lydia Becker Institute of Immunology and Inflammation)

Date:	2020
Abstract:	Safety assessment of graphene-based materials (GBMs) including graphene oxide (GO) is essential for their safe use across many sectors of society. In particular, the link between specific material properties and biological effects needs to be further elucidated. Here, the effects of lateral dimensions of GO sheets in acute and chronic pulmonary responses after single intranasal instillation in mice are compared. Micrometer-sized GO induces stronger pulmonary inflammation than nanometer-sized GO, despite reduced translocation to the lungs. Genome-wide RNA sequencing also reveals distinct size-dependent effects of GO, in agreement with the histopathological results. Although large GO, but not the smallest GO, triggers the formation of granulomas that persists for up to 90 days, no pulmonary fibrosis is observed. These latter results can be partly explained by Raman imaging, which evidences the progressive biotransformation of GO into less graphitic structures. The findings demonstrate that lateral dimensions play a fundamental role in the pulmonary response to GO, and suggest that airborne exposure to micrometer-sized GO should be avoided in the production plant or applications, where aerosolized dispersions are likely to occur. These results are important toward the implementation of a safer-by-design approach for GBM products and applications, for the benefit of workers and end-users.
Grants:	European Commission 696656
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Graphene oxide ; Inflammation ; Lung ; Macrophages ; Mice ; RNA sequencing
Published in:	Advanced science, Vol. 7, issue 12 (june 2020) , art. 1903200, ISSN 2198-3844

DOI: 10.1002/advs.201903200
PMID: 32596109

17 p, 13.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles