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Pàgina inicial > Articles > Articles publicats > In Vivo Bactericidal Efficacy of GWH1 Antimicrobial Peptide Displayed on Protein Nanoparticles, a Potential Alternative to Antibiotics |
Data: | 2020 |
Resum: | Oligomerization of antimicrobial peptides into nanosized supramolecular complexes produced in biological systems (inclusion bodies and self-assembling nanoparticles) seems an appealing alternative to conventional antibiotics. In this work, the antimicrobial peptide, GWH1, was N-terminally fused to two different scaffold proteins, namely, GFP and IFN-γ for its bacterial production in the form of such recombinant protein complexes. Protein self-assembling as regular soluble protein nanoparticles was achieved in the case of GWH1-GFP, while oligomerization into bacterial inclusion bodies was reached in both constructions. Among all these types of therapeutic proteins, protein nanoparticles of GWH1-GFP showed the highest bactericidal effect in an in vitro assay against Escherichia coli, whereas non-oligomerized GWH1-GFP and GWH1-IFN-γ only displayed a moderate bactericidal activity. These results indicate that the biological activity of GWH1 is specifically enhanced in the form of regular multi-display configurations. Those in vitro observations were fully validated against a bacterial infection using a mouse mastitis model, in which the GWH1-GFP soluble nanoparticles were able to effectively reduce bacterial loads. |
Ajuts: | Ministerio de Economía y Competitividad RTA2015-00064-C02-02 Ministerio de Economía y Competitividad RTA2015-00064-C02-01 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Mouse mastitis model ; Antimicrobial peptide ; Protein nanoparticle ; Inclusion body ; Recombinant protein ; Escherichia coli ; Staphylococcus aureus ; Therapeutic protein |
Publicat a: | Pharmaceutics, Vol. 12, Num. 12 (December 2020) , art. 1217, ISSN 1999-4923 |
16 p, 3.6 MB |