Identification of key sequence features required for microRNA biogenesis in plants
Rojas, Arantxa M. L. (Instituto de Biología Molecular y Celular de Rosario)
Drusin, Salvador I. (Instituto de Biología Molecular y Celular de Rosario)
Chorostecki, Uciel Pablo (Institut de Recerca Biomèdica)
Mateos, Julieta Lisa (Instituto de Fisiología, Biología Molecular y Neurociencias)
Moro, Belen (Centre de Recerca en Agrigenòmica)
Bologna, Nicolás (Centre de Recerca en Agrigenòmica)
Bresso, Edgardo G. (Instituto de Biología Molecular y Celular de Rosario)
Schapire, Arnaldo (Instituto de Biología Molecular y Celular de Rosario)
Rasia, Rodolfo (Instituto de Biología Molecular y Celular de Rosario)
Moreno, Diego M. (Universidad Nacional de Rosario. Departamento de Química-Física)
Palatnik, Javier (Instituto de Biología Molecular y Celular de Rosario)

Date:	2020
Abstract:	MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Arabidopsis ; Arabidopsis Proteins ; Base Pair Mismatch ; Cell Cycle Proteins ; Magnoliopsida ; MicroRNAs ; Molecular Dynamics Simulation ; Nucleic Acid Conformation ; Polymorphism, Single Nucleotide ; Ribonuclease III ; RNA Processing, Post-Transcriptional ; RNA, Plant
Published in:	Nature communications, Vol. 11 (October 2020) , art. 5320, ISSN 2041-1723

DOI: 10.1038/s41467-020-19129-6
PMID: 33087730

11 p, 3.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles