Auxin-Regulated Reversible Inhibition of TMK1 Signaling by MAKR2 Modulates the Dynamics of Root Gravitropism
Marques-Bueno, Mar (Centre de Recerca en Agrigenòmica)
Armengot, Laia (Université de Lyon. Laboratoire Reproduction et Développement des Plantes)
Noack, Lise C. (Université de Lyon. Laboratoire Reproduction et Développement des Plantes)
Bareille, Joseph (Université de Lyon. Laboratoire Reproduction et Développement des Plantes)
Rodriguez, Lesia (Institute of Science and Technology Austria)
Platre, Matthieu Pierre (Université de Lyon. Laboratoire Reproduction et Développement des Plantes)
Bayle, Vincent (Université de Lyon. Laboratoire Reproduction et Développement des Plantes)
Liu, Mengying (Université de Lyon. Laboratoire Reproduction et Développement des Plantes)
Opdenacker, Davi (Ghent University. Department of Plant Biotechnology and Bioinformatics)
Vanneste, Steffen (Lab of Plant Growth Analysis. Ghent University Global Campus)
Möller, Barbara K. (Department of Plant Biotechnology and Bioinformatics. Ghent University)
Nimchuk, Zachary L. (Department of Biology. University of North Carolina at Chapel Hill)
Beeckman, Tom (Ghent University. Department of Plant Biotechnology and Bioinformatics)
Caño Delgado, Ana I (Centre de Recerca en Agrigenòmica)
Friml, Jirí (Institute of Science and Technology Austria)
Jaillais, Yvon (Université de Lyon. Laboratoire Reproduction et Développement des Plantes)

Date:	2021
Abstract:	Plants are able to orient their growth according to gravity, which ultimately controls both shoot and root architecture. 1 Gravitropism is a dynamic process whereby gravistimulation induces the asymmetric distribution of the plant hormone auxin, leading to asymmetric growth, organ bending, and subsequent reset of auxin distribution back to the original pre-gravistimulation situation. 1, 2, 3 Differential auxin accumulation during the gravitropic response depends on the activity of polarly localized PIN-FORMED (PIN) auxin-efflux carriers. 1, 2, 3, 4 In particular, the timing of this dynamic response is regulated by PIN2,5,6 but the underlying molecular mechanisms are poorly understood. Here, we show that MEMBRANE ASSOCIATED KINASE REGULATOR2 (MAKR2) controls the pace of the root gravitropic response. We found that MAKR2 is required for the PIN2 asymmetry during gravitropism by acting as a negative regulator of the cell-surface signaling mediated by the receptor-like kinase TRANSMEMBRANE KINASE1 (TMK1). 2,7, 8, 9, 10 Furthermore, we show that the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself, which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner. Our findings suggest that the timing of the root gravitropic response is orchestrated by the reversible inhibition of the TMK1 signaling pathway at the cell surface.
Grants:	European Commission 683163 Ministerio de Economía y Competitividad BIO2016-78955 European Commission 3363360
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Brassinosteroid ; Gravitropism ; Receptor-like kinase ; Root ; TMK ; ROP6 ; MAKR ; Anionic lipids
Published in:	Current Biology, Vol. 31, Issue 1 (January 2021) , p. 228-237.e10, ISSN 1879-0445

DOI: 10.1016/j.cub.2020.10.011
PMID: 33157019

29 p, 4.3 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles