Several geranylgeranyl diphosphate synthase isoforms supply metabolic substrates for carotenoid biosynthesis in tomato
Barja, Maria Victoria (Centre de Recerca en Agrigenòmica)
Ezquerro, Miguel (Centre de Recerca en Agrigenòmica)
Beretta, Stefano (Centre de Recerca en Agrigenòmica)
Diretto, Gianfranco (Italian National Agency for New Technologies, Energy, and Sustainable Development (Roma, Itàlia))
Florez-Sarasa, Igor (Centre de Recerca en Agrigenòmica)
Feixes Prats, Elisenda (Centre de Recerca en Agrigenòmica)
Fiore, Alessia (Italian National Agency for New Technologies, Energy, and Sustainable Development (Roma, Itàlia))
Karlova, Rumyana (Wageningen University and Research)
Fernie, Alisdair (Max-Planck-Institut für Molekulare Pflanzenphysiologie)
Beekwilder, Jules (Wageningen University and Research)
Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)

Fecha:	2021
Resumen:	Geranylgeranyl diphosphate (GGPP) produced by GGPP synthase (GGPPS) serves as a precursor for many plastidial isoprenoids, including carotenoids. Phytoene synthase (PSY) converts GGPP into phytoene, the first committed intermediate of the carotenoid pathway. Here we used biochemical, molecular, and genetic tools to characterise the plastidial members of the GGPPS family in tomato (Solanum lycopersicum) and their interaction with PSY isoforms. The three tomato GGPPS isoforms found to localise in plastids (SlG1, 2 and 3) exhibit similar kinetic parameters. Gene expression analyses showed a preferential association of individual GGPPS and PSY isoforms when carotenoid biosynthesis was induced during root mycorrhization, seedling de-etiolation and fruit ripening. SlG2, but not SlG3, physically interacts with PSY proteins. By contrast, CRISPR-Cas9 mutants defective in SlG3 showed a stronger impact on carotenoid levels and derived metabolic, physiological and developmental phenotypes compared with those impaired in SlG2. Double mutants defective in both genes could not be rescued. Our work demonstrates that the bulk of GGPP production in tomato chloroplasts and chromoplasts relies on two cooperating GGPPS paralogues, unlike other plant species such as Arabidopsis thaliana, rice or pepper, which produce their essential plastidial isoprenoids using a single GGPPS isoform.
Ayudas:	Ministerio de Ciencia e Innovación BIO2017-84041-P Ministerio de Ciencia e Innovación BIO2017-90877-REDT Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-710 European Commission 753301 Ministerio de Economía y Competitividad PCIN-2015-103 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Educación, Cultura y Deporte FPU14/05142 Ministerio de Economía y Competitividad BES-2017-080652
Nota:	Altres ajuts: ERA-IB-2 (Industrial Biotechnology) BioProMo project, (PCIN-2015-103), EU-H2020 COST Action CA15136 (EuroCaroten)
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Carotenoids ; Geranylgeranyl diphosphate (GGPP) ; Prenyltransferase ; Ripening ; Synthase ; Tomato
Publicado en:	The new phytologist, (March 2021) , ISSN 1469-8137

DOI: 10.1111/nph.17283

Postprint 42 p, 19.8 MB

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