The Analysis of Live-Attenuated Piscirickettsia salmonis Vaccine Reveals the Short-Term Upregulation of Innate and Adaptive Immune Genes in Atlantic Salmon (Salmo salar) : an in situ open-sea cages study
Vargas, Deborah (Universidad de Santiago de Chile)
Reyes-Cerpa, Sebastián (Universidad Mayor)
Oyarzún-Arrau, Aarón (Ictio Biotechnologies S.A. Consorcio Tecnológico de Sanidad Acuícola)
Acuña-Castillo, Claudio (Universidad de Santiago de Chile)
Imarai, Mónica (Universidad de Santiago de Chile)
Reyes-López, Felipe E. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Sandino, Ana María (Universidad de Santiago de Chile)
Vallejos Vidal, Eva Carolina (Universidad de Santiago de Chile)

Data:	2021
Resum:	Piscirickettsia salmonis, the etiological agent of the Salmon Rickettsial Septicemia (SRS), is one the most serious health problems for the Chilean salmon industry. Typical antimicrobial strategies used against P. salmonis include antibiotics and vaccines, but these applications have largely failed. A few years ago, the first attenuated-live vaccine against SRS (ALPHA JECT LiVac® SRS vaccine) was released to the market. However, there is no data about the agents involved in the activation of the immune response induced under field conditions. Therefore, in this study we evaluated the expression profile of a set of gene markers related to innate and adaptive immunity in the context of a cellular response in Atlantic salmon (Salmo salar) reared under productive farm conditions and immunized with a live-attenuated vaccine against P. salmonis. We analyzed the expression at zero, 5-, 15- and 45-days post-vaccination (dpv). Our results reveal that the administration of the attenuated live SRS LiVac vaccine induces a short-term upregulation of the cellular-mediated immune response at 5 dpv modulated by the upregulation of ifnα, ifnγ, and the cd4 and cd8α T cell surface markers. In addition, we also registered the upregulation of il-10 and tgfβ. Altogether, the results suggest that a balanced activation of the immune response took place only at early times post-vaccination (5 dpv). The scope of this short-term upregulation of the cellular-mediated immune response against a natural outbreak in fish subjected to productive farm conditions deserves further research.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Piscirickettsia salmonis ; Salmonid Rickettsial Septicemia (SRS) ; Attenuated live vaccine ; Cellular immunity ; Interferon-mediated immune response ; Open sea cage farming
Publicat a:	Microorganisms, Vol. 9, Núm. 4 (March 2021) , art. 703, ISSN 2076-2607

DOI: 10.3390/microorganisms9040703
PMID: 33805284

12 p, 728.9 KB

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