The hCOMET project : International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
Milić, Mirta (Institute for Medical Research and Occupational Health, Croatia)
Ceppi, Marcello (San Martino Policlinic Hospital, Italy)
Bruzzone, Marco (San Martino Policlinic Hospital, Italy)
Azqueta, Amaya (Instituto de Investigación Sanitaria de Navarra)
Brunborg, Gunnar (Norwegian Institute of Public Health)
Godschalk, Roger (University of Maastricht)
Koppen, Gudrun (Flemish Institute of Technological Research, Environmental Risk and Health unit VITO - BIOMo, Belgium)
Langie, Sabine (University of Maastricht)
Møller, Peter (University of Copenhagen)
Teixeira, João Paulo (Universidade do Porto)
Alija, Avdulla (University of Prishtina, Kosovo)
Anderson, Diana (University of Bradford)
Andrade, Vanessa (University of Southern Santa Catarina, Brazil)
Andreoli, Cristina (Istituto Superiore di Sanità)
Asllani, Fisnik (University of Prishtina, Kosovo)
Bangkoglu, Ezgi Eyluel (University of Wuerzburg)
Barančoková, Magdalena (Slovak Medical University)
Basaran, Nursen (Hacettepe University, Turkey)
Boutet-Robinet, Elisa (Université de Toulouse)
Buschini, Annamaria (University of Parma)
Cavallo, Delia (Italian Workers' Compensation Authority (INAIL))
Pereira, Cristiana Costa (Universidade do Porto)
Costa, Carla (Universidade do Porto)
Costa, Solange (Universidade do Porto)
Da Silva, Juliana (Lutheran University of Brazil (ULBRA))
Del Bo', Cristian (University of Milan)
Srećković, Vesna Dimitrijević (University of Belgrade)
Djelić, Ninoslav (University of Belgrade)
Dobrzyńska, Malgorzata (National Institute of Public Health - National Institute of Hygiene, Poland)
Duračková, Zdenka (Comenius University, Slovakia)
Dvořáková, Monika (Comenius University, Slovakia)
Gajski, Goran (Institute for Medical Research and Occupational Health, Croatia)
Galati, Serena (University of Parma)
Lima, Omar García (Center for RadiationProtection and Hygiene, Cuba)
Giovannelli, Lisa (University of Florence)
Goroshinskaya, Irina A. (National Medical Research Center for Oncology, Russia)
Grindel, Annemarie (University of Vienna)
Gutzkow, Kristine B. (Norwegian Institute of Public Health (NIPH))
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández, Carlos (Instituto de Ciencias Básicas y Preclínicas "Victoria de Giron", Cuba)
Holven, Kirsten B. (University of Oslo)
Ibero-Baraibar, Idoia (Universidad Pública de Navarra)
Ottestad, Inger (University of Oslo)
Kadioglu, Ela (Gazi University, Turkey)
Kažimirová, Alena (Slovak Medical University)
Kuznetsova, Elena (Russian Academy of Sciences)
Ladeira, Carina (Universidade NOVA de Lisboa)
Lamonaca, Palma (San Raffaele University)
Lebailly, Pierre (Centre François Baclesse (Caen, França))
Louro, Henriqueta (ToxOmics, NMS, NOVA University of Lisbon, Lisbon, Portugal)
Cardoso, Tania Mandina (Center for RadiationProtection and Hygiene, Cuba)
Marcon, Francesca (Istituto Superiore di Sanità)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Moretti, Massimo (University of Perugia)
Moretti, Silvia (Palagi Hospital, Italy)
Najafzadeh, Mojgan (University of Bradford)
Nemeth, Zsuzsanna (National Public Health CenterHungary)
Neri, Monica (San Raffaele University)
Novotna, Bozena (The Czech Academy of Sciences)
Orlow, Irene (Memorial Sloan Kettering Cancer Center, Epidemiology and Biostatistics,USA)
Paduchova, Zuzana (Comenius University, Slovakia)
Pastor, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Perdry, Hervé (Univ Paris-Saclay)
Spremo-Potparević, Biljana (University of Belgrade)
Ramadhani, Dwi (National Nuclear Energy Agency of Indonesia)
Riso, Patrizia (University of Milan)
Rohr, Paula (University of Southern Santa Catarina, Brazil)
Rojas, Emilio (Universidad Nacional Autónoma de México)
Rossner, Pavel (The Czech Academy of Sciences)
Safar, Anna (National Public Health Center, Hungary)
Sardas, Semra (Istinye University, Turkey)
Silva, Maria João (University of Lisbon)
Sirota, Nikolay (Russian Academy of Sciences)
Smolkova, Bozena (Slovak Academy of Sciences)
Staruchova, Marta (Slovak Medical University)
Stetina, Rudolf (University of Defence, Czech Republic)
Stopper, Helga (University of Wuerzburg,Germany)
Surikova, Ekaterina I. (National Medical Research Center for Oncology, Russia)
Ulven, Stine M. (University of Oslo)
Ursini, Cinzia Lucia (Italian Workers' Compensation Authority (INAIL))
Valdiglesias, Vanessa (Universidade da Coruña)
Valverde, Mahara (Universidad Nacional Autónoma de México)
Vodicka, Pavel (Experimental Medicine, Molecular Biology of Cancer, IEM AVCR, Czech Republic)
Volkovova, Katarina (Slovak Medical University)
Wagner, Karl-Heinz (University of Vienna)
Živković, Lada (University of Belgrade)
Dušinská, Maria (NILU, Health Effects Laboratory, Norway)
Collins, Andrew R. (University of Oslo)
Bonassi, Stefano (San Raffaele University)

Date:	2021
Abstract:	The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
Grants:	Ministerio de Economía y Competitividad CB12/03/30002 Ministerio de Economía y Competitividad RYC-2013-14370
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Comet assay ; DNA damage ; Pooled analysis ; Human biomonitoring ; Biomarkers
Published in:	Mutation research. Reviews in mutation research, Vol. 787 (February 2021) , art. 108371, ISSN 1388-2139

DOI: 10.1016/j.mrrev.2021.108371
PMID: 34083035

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