Identification of RipAZ1 as an avirulence determinant of Ralstonia solanacearum in Solanum americanum
Moon, Hayoung (Pohang University of Science and Technology. Department of Life Sciences)
Pandey, Ankita (Pohang University of Science and Technology. Department of Life Sciences)
Yoon, Hayeon (Pohang University of Science and Technology. Department of Life Sciences)
Choi, Sera (Pohang University of Science and Technology. Department of Life Sciences)
Jeon, Hyelim (Seoul National University. Department of Agriculture, Forestry and Bioresources)
Prokchorchik, Maxim (Pohang University of Science and Technology. Department of Life Sciences)
Jung, Gayoung (Pohang University of Science and Technology. Department of Life Sciences)
Witek, Kamil (University of East Anglia. The Sainsbury Laboratory)
Valls, Marc (Centre de Recerca en Agrigenòmica)
McCann, Honour C. (Max Planck Institute for Developmental Biology)
Kim, Min-Sung (Pohang University of Science and Technology. Department of Life Sciences)
Jones, Jonathan D. G. (University of East Anglia. The Sainsbury Laboratory)
Segonzac, Cécile (Seoul National University. Agricultural Life Science Research Institute)
Sohn, Kee Hoon (Pohang University of Science and Technology. School of Interdisciplinary Bioscience and Bioengineering)

Data:	2021
Resum:	Ralstonia solanacearum causes bacterial wilt disease in many plant species. Type III-secreted effectors (T3Es) play crucial roles in bacterial pathogenesis. However, some T3Es are recognized by corresponding disease resistance proteins and activate plant immunity. In this study, we identified the R. solanacearum T3E protein RipAZ1 (Ralstonia injected protein AZ1) as an avirulence determinant in the black nightshade species Solanum americanum. Based on the S. americanum accession-specific avirulence phenotype of R. solanacearum strain Pe_26, 12 candidate avirulence T3Es were selected for further analysis. Among these candidates, only RipAZ1 induced a cell death response when transiently expressed in a bacterial wilt-resistant S. americanum accession. Furthermore, loss of ripAZ1 in the avirulent R. solanacearum strain Pe_26 resulted in acquired virulence. Our analysis of the natural sequence and functional variation of RipAZ1 demonstrated that the naturally occurring C-terminal truncation results in loss of RipAZ1-triggered cell death. We also show that the 213 amino acid central region of RipAZ1 is sufficient to induce cell death in S. americanum. Finally, we show that RipAZ1 may activate defence in host cell cytoplasm. Taken together, our data indicate that the nucleocytoplasmic T3E RipAZ1 confers R. solanacearum avirulence in S. americanum. Few avirulence genes are known in vascular bacterial phytopathogens and ripAZ1 is the first one in R. solanacearum that is recognized in black nightshades. This work thus opens the way for the identification of disease resistance genes responsible for the specific recognition of RipAZ1, which can be a source of resistance against the devastating bacterial wilt disease. Ralstonia solanacearum type III effector RipAZ1 confers bacterial avirulence during interaction with a wild plant species Solanum americanum.
Ajuts:	Agencia Estatal de Investigación PID2019-108595RB-I00 Ministerio de Economía y Competitividad SEV/2015/0533
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Avirulence ; Bacterial wilt ; Effector ; Effector-triggered immunity ; Ralstonia solanacearum ; Solanum americanum
Publicat a:	Molecular plant pathology, Vol. 22, Issue 3 (March 2021) , p. 317-333, ISSN 1364-3703

DOI: 10.1111/mpp.13030
PMID: 33389783

17 p, 1.4 MB

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