The microbiota of the surface, dermis and subcutaneous tissue of dog skin
García Fonticoba, Rocío (Universitat Autònoma de Barcelona. Fundació Hospital Clínic Veterinari)
Ferrer i Caubet, Lluís (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Francino, Olga (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Cuscó, Anna (Universitat Autònoma de Barcelona. Vetgenomics)

Fecha:	2020
Resumen:	Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of dogs without cutaneous disease using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS). Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks. When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue.
Ayudas:	Ministerio de Ciencia e Innovación RTI2018-101991-B-I00
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Skin microbiota ; Epidermis ; Dermis ; Subcutaneous tissue ; Next-generation sequencing ; Dog
Publicado en:	Animal Microbiome, Vol. 2 (september 2020) , ISSN 2524-4671

DOI: 10.1186/s42523-020-00050-8
PMID: 33499979

9 p, 1.2 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
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