HVCN1 Channels Are Relevant for the Maintenance of Sperm Motility During In Vitro Capacitation of Pig Spermatozoa
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
Llavanera, Marc (Universitat de Girona. Departament de Biologia)
Mateo-Otero, Yentel (Universitat de Girona. Departament de Biologia)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Bonet, Sergi (Universitat de Girona. Departament de Biologia)
Pinart, Elisabeth (Universitat de Girona. Departament de Biologia)

Data:	2020
Resum:	The objective of the present study was to determine the physiological role of voltage-gated hydrogen channels 1 (HVCN1 channels) during in vitro capacitation of pig spermatozoa. Sperm samples from 20 boars were incubated in capacitating medium for 300 minutes (min) in the presence of 2-guanidino benzimidazole (2-GBI), a specific HVCN1-channel blocker, added either at 0 min or after 240 min of incubation. Control samples were incubated in capacitating medium without the inhibitor. In all samples, acrosomal exocytosis was triggered with progesterone after 240 min of incubation. Sperm viability, sperm motility and kinematics, acrosomal exocytosis, membrane lipid disorder, intracellular calcium levels and mitochondrial membrane potential were evaluated after 0, 60, 120, 180, 240, 250, 270 and 300 min of incubation. While HVCN1-blockage resulted in altered sperm viability, sperm motility and kinematics and reduced mitochondrial membrane potential as compared to control samples, at any blocker concentration and incubation time, it had a non-significant effect on intracellular Ca 2+ levels determined through Fluo3-staining. The effects on acrosomal exocytosis were only significant in blocked samples at 0 min, and were associated with increased membrane lipid disorder and Ca 2+ levels of the sperm head determined through Rhod5-staining. In conclusion, HVCN1 channels play a crucial role in the modulation of sperm motility and kinematics, and in Ca 2+ entrance to the sperm head.
Ajuts:	Ministerio de Ciencia e Innovación RYC-2014-15581 Ministerio de Ciencia e Innovación AGL2017-88329-R Ministerio de Ciencia e Innovación FPU18/00666
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	In vitro capacitation ; Boar sperm ; HVCN1 channels ; 2-GBI ; Progesterone
Publicat a:	International journal of molecular sciences, Vol. 21 (may 2020) , ISSN 1422-0067

DOI: 10.3390/ijms21093255
PMID: 32375375

22 p, 5.3 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats