Selection of Immature Cat Oocytes with Brilliant Cresyl Blue Stain Improves In Vitro Embryo Production during Non-Breeding Season
Piras, Anna Rita (Università degli Studi di Sassari. Dipartimento di Medicina Veterinaria)
Ariu, Federica (Università degli Studi di Sassari. Dipartimento di Medicina Veterinaria)
Zedda, Maria-Teresa (Università degli Studi di Sassari. Dipartimento di Medicina Veterinaria)
Paramio, María Teresa (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Bogliolo, Luisa (Università degli Studi di Sassari. Dipartimento di Medicina Veterinaria)

Data:	2020
Resum:	The domestic cat is commonly used as a model for the development of assisted reproductive technologies, including in vitro embryo production (IVEP) in felid species. Seasonal reproduction is a feature of domestic cats as well as of several species of wild feline. Likewise, the number and the quality of blastocysts produced in in vitro systems is linked to season. Maintaining stable in vitro embryo production throughout the year is crucial not only for research purposes but also for programs aimed at protecting endangered felines. We assess whether using Brilliant Cresyl Blue (BCB) selection in addition to the classical morphological selection could improve the IVEP outcomes during non-breeding season. Blastocyst yield and quality of the embryos (hatching rate and blastocyst cell numbers) were higher after IVM/IVF in oocytes defined as BCB+ (colored cytoplasm) based on the BCB test than in oocytes only morphologically selected. Furthermore, no adverse effects on bioenergetic/oxidative status were observed in oocytes subjected to BCB staining. In conclusion, BCB test implementation in IVEP programs might ensure a steady output of domestic cat blastocysts throughout the year. In domestic cats, the maturation, fertilization, and development potential in vitro decreases during the non-breeding season. This study aims at evaluating the efficacy of Brilliant Cresyl Blue (BCB) staining in selecting developmentally competent oocytes to be used in in vitro embryo production (IVEP) programs in order to overcome the season variability in blastocyst yield. Cumulus-oocytes complexes (COCs) collected from antral follicles of domestic cat ovaries during the anestrus phase (July to November) were selected by BCB staining and classified as BCB+ (colored cytoplasm) and BCB− (colorless cytoplasm). COCs not exposed to BCB staining were used as control. Before and after in vitro maturation mitochondrial activity and reactive oxygen species (ROS) were measured. Following in vitro fertilization, blastocyst rate, hatching rate, and blastocyst cell numbers were recorded. The results show that BCB staining did not alter the mitochondrial function and ROS production in cat oocytes. BCB+ oocytes presented a higher (p < 0. 05) blastocyst rate, hatching rate, and blastocyst cell number than BCB− and control oocytes. In conclusion, BCB staining does not affect the bioenergetic/oxidative status of the oocyte while being a useful tool for selecting good quality oocytes to increase IVEP in domestic cats during non-breeding season.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	BCB ; Blastocyst ; Domestic cat ; In vitro maturation ; Mitochondria ; ROS ; Seasonality
Publicat a:	Animals, Vol. 10 (august 2020) , ISSN 2076-2615

DOI: 10.3390/ani10091496
PMID: 32847086

12 p, 1.3 MB

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