Chitosan Induces Plant Hormones and Defenses in Tomato Root Exudates
Suarez-Fernandez, Marta (University of Alicante. Department of Marine Sciences and Applied Biology)
Marhuenda-Egea, Frutos Carlos (University of Alicante. Department of Agrochemistry and Biochemistry)
Lopez-Moya, Federico (University of Alicante. Department of Marine Sciences and Applied Biology)
Arnao, Marino B. (University of Murcia. Department of Plant Biology)
Cabrera-Escribano, Francisca (University of Seville. Department of Organic Chemistry)
Nueda, Maria Jose (University of Alicante. Department of Mathematics)
Gunsé Forcadell, Benito (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Lopez-Llorca, Luis Vicente (University of Alicante. Department of Marine Sciences and Applied Biology)

Date:	2020
Abstract:	In this work, we use electrophysiological and metabolomic tools to determine the role of chitosan as plant defense elicitor in soil for preventing or manage root pests and diseases sustainably. Root exudates include a wide variety of molecules that plants and root microbiota use to communicate in the rhizosphere. Tomato plants were treated with chitosan. Root exudates from tomato plants were analyzed at 3, 10, 20, and 30 days after planting (dap). We found, using high performance liquid chromatography (HPLC) and excitation emission matrix (EEM) fluorescence, that chitosan induces plant hormones, lipid signaling and defense compounds in tomato root exudates, including phenolics. High doses of chitosan induce membrane depolarization and affect membrane integrity. 1 H-NMR showed the dynamic of exudation, detecting the largest number of signals in 20 dap root exudates. Root exudates from plants irrigated with chitosan inhibit ca. twofold growth kinetics of the tomato root parasitic fungus Fusarium oxysporum f. sp. radicis-lycopersici. and reduced ca. 1. 5-fold egg hatching of the root-knot nematode Meloidogyne javanica.
Grants:	Ministerio de Economía y Competitividad AGL2015-66833-R European Commission 727624
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Chitosan ; Root exudates ; Membrane potential ; Lipid signaling ; Plant defenses ; Plant hormones ; Soil-borne pathogens
Published in:	Frontiers in plant science, Vol. 11 (November 2020) , art. 572087, ISSN 1664-462X

DOI: 10.3389/fpls.2020.572087
PMID: 33250907

15 p, 3.7 MB

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