Changes in Acute Phase Proteins in Bitches after Laparoscopic, Midline, and Flank Ovariectomy Using the Same Method for Hemostasis
Del Romero, Ayla (Universidad CEU Cardenal Herrera)
Cuervo, Belén (Universidad CEU Cardenal Herrera)
Peláez, Pau (Universidad CEU Cardenal Herrera)
Miguel, Laura (Universidad CEU Cardenal Herrera)
Torres-Torrillas, Marta (Universidad CEU Cardenal Herrera)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)
Rivera del Álamo, Maria Montserrat (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rubio, Camila Peres (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Rubio, Mónica (Universidad CEU Cardenal Herrera)

Data:	2020
Resum:	Ovariectomy is a common surgical procedure in veterinary medicine, with many techniques involved. The aim of this study is to objectively evaluate the acute phase response by measuring the changes in a panel of acute phase proteins after applying three different ovariectomy techniques. C-reactive protein values showed increases of lower magnitude after laparoscopic ovariectomy compared with other techniques, indicating that this surgical technique induces a reduced inflammatory response and tissue damage. The use of this procedure is in agreement with the current tendency to use minimally invasive procedures for ovariectomy. Acute phase proteins (APP) are biomarkers of systemic inflammation, which allow monitoring the evolution of diseases, the response to treatments, and post-operative complications. Ovariectomy (OVE) is frequently performed in veterinary medicine and can be a useful model to evaluate surgical trauma and inflammation in the bitch. The objective was to investigate and compare the acute phase response (APR) after applying three different OVE techniques by measuring serum levels of C-reactive protein (CRP), haptoglobin (Hp), albumin (Alb), and paraoxonase-1 (PON-1). Forty-five intact bitches were included in the study, being randomly distributed into three groups: laparoscopic OVE (L), midline OVE (M), and flank OVE (F). Serum CRP, Hp, Alb, and PON-1 were measured before surgery, 1, 24, 72, and 168 h post-intervention. CRP levels increased significantly 24 h post-surgery in the M and F groups, but no significant variation was observed in the L group at any time of the study period. Hp was significantly higher in group L than in group F 72 h post-surgery. Alb and PON-1 showed no statistical difference among groups or among sampling periods. CRP response suggests that the use of laparoscopic procedures produce lower inflammation compared to open conventional approaches when performing OVE in the bitch.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Acute phase proteins ; Surgical trauma ; Ovariectomy ; Laparoscopy
Publicat a:	Animals, Vol. 10 (november 2020) , ISSN 2076-2615

Adreça alternativa: https://www.mdpi.com/2076-2615/10/12/2223
DOI: 10.3390/ani10122223
PMID: 33260846

14 p, 1.9 MB

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