Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light
Catalán, Jaime (Universitat de Girona. Departament de Biologia)
Papas, Marion (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Trujillo-Rojas, Lina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Blanco Prieto, Olga (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Bonilla Correal, Sebastian (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Rodríguez-Gil, Joan E. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Yeste Oliveras, Marc (Universitat de Girona. Departament de Biologia)

Data:	2020
Resum:	This work aimed to investigate how stimulation of donkey sperm with red LED light affects mitochondrial function. For this purpose, freshly diluted donkey semen was stimulated with red light for 1, 5, and 10 min, in the presence or absence of oligomycin A (Omy A), a specific inhibitor of mitochondrial ATP synthase, or FCCP, a specific disruptor of mitochondrial electron chain. The results obtained in the present study indicated that the effects of red LED light on fresh donkey sperm function are related to changes in mitochondria function. In effect, irradiation of donkey sperm resulted in an increase in mitochondrial membrane potential (MMP), the activity of cytochrome C oxidase and the rate of oxygen consumption. In addition, in the absence of oligomycin A and FCCP, light-stimulation augmented the average path velocity (VAP) and modified the structure of motile sperm subpopulations, increasing the fastest and most linear subpopulation. In contrast, the presence of either Omy A or FCCP abolished the aforementioned effects. Interestingly, our results also showed that the effects of red light depend on the exposure time applied, as indicated by the observed differences between irradiation protocols. In conclusion, our results suggest that exposing fresh donkey sperm to red light modulates the function of their mitochondria through affecting the activity of the electron chain. However, the extent of this effect depends on the irradiation pattern and does not exclude the existence of other mechanisms, such as those related to thermotaxis.
Ajuts:	Agencia Estatal de Investigación AGL2017-88329-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Sperm ; Red light stimulation ; Mitochondrial function ; Donkey ; Oligomycin A ; FCCP
Publicat a:	Frontiers in Cell and Developmental Biology, Vol. 8 (december 2020) , ISSN 2296-634X

DOI: 10.3389/fcell.2020.588621
PMID: 33365309

18 p, 2.2 MB

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