PrimeStore MTM and OMNIgene Sputum for the Preservation of Sputum for Xpert MTB/RIF Testing in Nigeria
Bimba, John (Bingham University (Nigèria))
Lawson, Lovett (Bingham University (Nigèria))
Kontogianni, Konstantina (Liverpool School of Tropical Medicine)
Edwards, Thomas (Liverpool School of Tropical Medicine)
Ekpenyong, Bassey Emanna (Bingham University (Nigèria))
Dodd, James (Liverpool School of Tropical Medicine)
Adams, Emily (Liverpool School of Tropical Medicine)
Sloan, Derek J. (University of St Andrews, UK)
Creswell, Jacob (Stop TB Partnership, Geneva, Switzerland)
Domínguez, José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Cuevas, Luis E. (Liverpool School of Tropical Medicine)
Universitat Autònoma de Barcelona

Date:	2019
Abstract:	Background: Xpert MTB/RIF (GX) for tuberculosis (TB) diagnosis is often located in reference laboratories, and sputum needs to be transported using a cold chain. Transport media to preserve sputum are available, but performance data under programmatic conditions are limited. Methods: Sputum samples were collected from patients with presumptive TB in Nigeria. One sputum was transported in a cold chain, tested immediately with GX and cultured. One sputum was swabbed and stored in PrimeStore-Molecular-Transport-Medium (Primestore), and the remainder was stored in OMNIGene-sputum (Omnigene), kept for seven days and tested with GX. Results: Of 248 patients, 63 were fresh-sputum culture-positive and 56 GX-positive (sensitivity 88. 9%, 95% CI: 78. 4-95. 4%). Four of 185 culture-negative patients were GX-positive (specificity 97. 8%, 94. 6-99. 4%). Omnigene GX and Primestore GX were positive in 56/62 (90. 3%, 80. 1-96. 4%) and 49/62 (79. 0%, 66. 8-88. 3%) culture-positive, respectively, and 1/185 (99. 5%, 97. 0-100. 0%) and 3/185 (98. 4%, 95. 3-99. 7%) were culture-negative patients. 14 Human Immunodeficiency Virus (HIV)-infected and 44 HIV-uninfected patients were culture-positive. Omnigene and Primestore detected 12/14 (85. 7%, 57. 2-98. 2%) and 5/14 (35. 7%, 12. 8-64. 9%) HIV-infected and 41/44 (93. 2%, 81. 3-98. 6%) HIV-uninfected culture-positive patients. Interpretation: Omnigene stored and fresh sputum samples had similar GX results. The GX results of Primestore-stored samples were similar to those found in the fresh sputum of non-HIV infected patients, but GX-positivity was lower in HIV-infected patients. This was likely due to the lower amount of bacilli collected by the swab and transferred to PrimeStore.
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Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Tuberculosis ; Diagnosis ; OMNIGene-sputum ; PrimeStore ; Xpert MTB/RIF
Published in:	Journal of clinical medicine, Vol. 8 (December 2019) , ISSN 2077-0383

DOI: 10.3390/jcm8122146
PMID: 31817201

8 p, 960.2 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles