Mycobacterium tuberculosis complex genotypes circulating in Nigeria based on spoligotyping obtained from Ziehl-Neelsen stained slides extracted DNA
Molina-Moya, Barbara (Institut Germans Trias i Pujol)
Gomgnimbou, Michel Kiréopori (Centre Muraz, and Faculté de Médecine, Bobo-Dioulasso, Burkina Faso)
Spinasse, Lizania (Université Paris Sud. Institut for Integrative Biology of the Cell)
Obasanya, Joshua (National TB, Buruli Ulcer and Leprosy Control Programme, Abuja, Nigeria)
Oladimeji, Olanrewaju (Zankli Medical Center, Abuja, Nigeria)
Dacombe, Russell (Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom)
Edwards, Thomas (Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom)
Daragon, Xavier-Olessa (Université Paris Sud. Institut for Integrative Biology of the Cell)
Lawson, Lovett (Bingham University, Nassarawa State, Nigeria)
Abdurrahman, Saddiq T. (National TB, Buruli Ulcer and Leprosy Control Programme, Abuja, Nigeria)
Cuevas, Luis E. (Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, United Kingdom)
Domínguez, José (Institut Germans Trias i Pujol)
Sola, Christophe (Université Paris Sud. Institut for Integrative Biology of the Cell)

Date:	2018
Abstract:	Methods: All State TB control programmes in Nigeria were requested to submit 25-50 smear-positive Ziehl-Neelsen (ZN) stained slides for screening during 2013-2014. DNA was extracted from 929 slides for spoligotyping and drug-resistance analysis using microbead-based flow-cytometry suspension arrays. Results: Spoligotyping results were obtained for 549 (59. 1%) of 929 samples. Lineage 4 Cameroon sublineage (L4. 6. 2) represented half of the patterns, Mycobacterium africanum (L5 and L6) represented one fifth of the patterns, and all other lineages, including other L4 sublineages, represented one third of the patterns. Sublineage L4. 6. 2 was mostly identified in the north of the country whereas L5 was mostly observed in the south and L6 was scattered. The spatial distribution of genotypes had genetic geographic gradients. We did not obtain results enabling the detection of drug-resistance mutations. Conclusion/significance: We present the first national snapshot of the M. tuberculosis spoligotypes circulating in Nigeria based on ZN slides. Spoligotyping data can be obtained in a rapid and high-throughput manner with DNA extracted from ZN-stained slides, which may potentially improve our understanding of the genetic epidemiology of TB.
Note:	Altres ajuts: This study was funded by an EDCTP grant attributed to LEC, Grant n SP.201141304.21. The study was funded by a Strategic Award grant from the European and Developing Countries Clinical Trial Partnership (grant SP.2011.41304.021) and its cofounders, the Medical Research Council UK and Instituto de Salud Carlos III-ISCIII Spain.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PLoS neglected tropical diseases, Vol. 12 (february 2018) , ISSN 1935-2735

DOI: 10.1371/journal.pntd.0006242
PMID: 29447161

13 p, 9.7 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles