Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk
Rubio, Mercedes (Hospital Clínic i Provincial de Barcelona)
Bustamante, Mariona (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Hernandez-Ferrer, Carles (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Fernandez-Orth, Dietmar (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Pantano, Lorena (Harvard T.H. Chan School of Public Health (Boston, Estats Units d'Amèrica))
Sarria, Yaris (Institut Hospital del Mar d'Investigacions Mèdiques)
Piqué-Borras, Maria (University Children's Hospital Basel (Basilea, Suïssa))
Vellve, Kilian (Hospital del Mar (Barcelona, Catalunya))
Agramunt Garcia, Sílvia (Hospital del Mar (Barcelona, Catalunya))
Carreras Collado, Ramón (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i Medicina Preventiva i Salut Pública)
Estivill, Xavier (Hospital Dexeus (Barcelona, Catalunya))
González, Juan Ramón (Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública)
Mayor, Alfredo (Hospital Clínic i Provincial de Barcelona)

Data:	2018
Resum:	Circulating small RNAs, including miRNAs but also isomiRs and other RNA species, have the potential to be used as non-invasive biomarkers for communicable and non-communicable diseases. This study aims to characterize and compare small RNA profiles in human biofluids. For this purpose, RNA was extracted from plasma and breast milk samples from 15 healthy postpartum mothers. Small RNA libraries were prepared with the NEBNext® small RNA library preparation kit and sequenced in an Illumina HiSeq2000 platform. miRNAs, isomiRs and clusters of small RNAs were annotated using seqBuster/seqCluster framework in 5 plasma and 10 milk samples that passed the initial quality control. The RNA yield was 81 ng/mL [standard deviation (SD): 41] and 3985 ng/mL (SD: 3767) for plasma and breast milk, respectively. Mean number of good quality reads was 4. 04 million (M) (40. 01% of the reads) in plasma and 12. 5M (89. 6%) in breast milk. One thousand one hundred eighty two miRNAs, 12,084 isomiRs and 1,053 small RNA clusters that included piwi-interfering RNAs (piRNAs), tRNAs, small nucleolar RNAs (snoRNA) and small nuclear RNAs (snRNAs) were detected. Samples grouped by biofluid, with 308 miRNAs, 1,790 isomiRs and 778 small RNA clusters differentially detected. In summary, plasma and milk showed a different small RNA profile. In both, miRNAs, piRNAs, tRNAs, snRNAs, and snoRNAs were identified, confirming the presence of non-miRNA species in plasma, and describing them for the first time in milk.
Ajuts:	Ministerio de Economía y Competitividad PI13/01478 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014SGR263
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	PloS one, Vol. 13 (march 2018) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0193527
PMID: 29505615

19 p, 2.5 MB

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