Microbead-based spoligotyping of Mycobacterium tuberculosis from Ziehl-Neelsen-stained microscopy preparations in Ethiopia
Molina-Moya, Barbara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Agonafir, Mulualem (LSTM, Reach Ethiopia)
Blanco, Silvia (Addis Ababa University. College of Natural Sciences)
Dacombe, Russell (Liverpool School of Tropical Medicine)
Gomgnimbou, Michel Kiréopori (Université Paris-Saclay)
Spinasse, Lizania (Université Paris-Saclay)
Gomes Fernandes, Meissiner (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Datiko, Daniel G. (REACH ETHIOPIA, Hawassa, Ethiopia)
Edwards, Thomas (Liverpool School of Tropical Medicine)
Cuevas, Luis E. (Liverpool School of Tropical Medicine)
Domínguez, José (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Sola, Christophe (Université Paris-Saclay)

Date:	2018
Abstract:	The worldwide dissemination of Mycobacterium tuberculosis strains has led to the study of their genetic diversity. One of the most used genotyping methods is spoligotyping, based on the detection of spacers in the clustered regularly interspaced short palindromic repeats (CRISPR) locus. This study assessed the performance of a microbead-based spoligotyping assay using samples extracted from Ziehl-Neelsen-stained smear-microscopy preparations and described the genetic diversity of Mycobacterium tuberculosis among new TB patients in Southern Nations, Nationalities and Peoples' Region (SNNPR) in Ethiopia. Among the 91 samples analysed, 59 (64. 8%) generated spoligotyping patterns. Fifty (84. 7%) samples were classified into 12 clusters (mostly Lineage 4 or 3) comprising 2-11 samples and nine had unique spoligotyping patterns. Among the 59 spoligotyping patterns, 25 belonged to the T1 sublineage, 11 to the T3-ETH, 5 to the URAL, 4 to the H3 and 14 to other L4 sublineages. There was a remarkable variation in genetic distribution in SNNPR compared to other regions of the country. Microbead-based spoligotyping is an easy-to-perform, high-throughput assay that can generate genotyping information using material obtained from smear microscopy preparations. The method provides an opportunity to obtain data of the M. tuberculosis genetic epidemiology in settings with limited laboratory resources.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 8 (march 2018) , ISSN 2045-2322

DOI: 10.1038/s41598-018-22071-9
PMID: 29507363

6 p, 1.6 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles