SETD7 Regulates the Differentiation of Human Embryonic Stem Cells
Castaño Cardoso, Julio (Centro de Medicina Regenerativa de Barcelona)
Morera, Cristina (Centro de Medicina Regenerativa de Barcelona)
Sesé, Borja (Centro de Medicina Regenerativa de Barcelona)
Boue, Stephanie (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Bonet-Costa, Carles (Institut de Biologia Molecular de Barcelona)
Martí, Merce (Centro de Medicina Regenerativa de Barcelona)
Roque Córdova, Alicia (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Jordan, Albert (Institut de Biologia Molecular de Barcelona)
Barrero, Maria J. (Centro Nacional de Investigaciones Oncológicas)

Date:	2016
Abstract:	The successful use of specialized cells in regenerative medicine requires an optimization in the differentiation protocols that are currently used. Understanding the molecular events that take place during the differentiation of human pluripotent cells is essential for the improvement of these protocols and the generation of high quality differentiated cells. In an effort to understand the molecular mechanisms that govern differentiation we identify the methyltransferase SETD7 as highly induced during the differentiation of human embryonic stem cells and differentially expressed between induced pluripotent cells and somatic cells. Knock-down of SETD7 causes differentiation defects in human embryonic stem cell including delay in both the silencing of pluripotency-related genes and the induction of differentiation genes. We show that SETD7 methylates linker histone H1 in vitro causing conformational changes in H1. These effects correlate with a decrease in the recruitment of H1 to the pluripotency genes OCT4 and NANOG during differentiation in the SETD7 knock down that might affect the proper silencing of these genes during differentiation.
Grants:	Ministerio de Educación y Ciencia RYC-2007-01510 Ministerio de Ciencia e Innovación BES-2008-009567 Ministerio de Economía y Competitividad PT13/0001/0041 Ministerio de Economía y Competitividad RD12/0019/0034 Ministerio de Ciencia e Innovación SAF2009-08588 Ministerio de Economía y Competitividad BFU2014-52237
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 11 (February 2016) , art. e0149502, ISSN 1932-6203

DOI: 10.1371/journal.pone.0149502
PMID: 26890252

21 p, 3.2 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles