Distinct functional enrichment of transcriptional signatures in pigs with high and low IFN-gamma responses after vaccination with a porcine reproductive and respiratory syndrome virus (PRRSV)
Ait-Ali, Tahar (University of Edinburgh. The Roslin Institute and Royal (Dick) School of Veterinary Studies)
Díaz, Ivan (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Soldevila, Ferran (Royal Veterinary College. Department of Pathology and Pathogen Biology)
Cano, Esmeralda (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Li, Yanli (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Wilson, Alison D. (University of Edinburgh. The Roslin Institute and Royal (Dick) School of Veterinary Studies)
Giotti, Bruno (University of Edinburgh. The Roslin Institute and Royal (Dick) School of Veterinary Studies)
Archibald, Alan L. (University of Edinburgh. The Roslin Institute and Royal (Dick) School of Veterinary Studies)
Mateu de Antonio, Enrique María (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Darwich Soliva, Laila (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Data:	2016
Resum:	The online version of this article (doi:10. 1186/s13567-016-0392-3) contains supplementary material, which is available to authorized users.
Ajuts:	Ministerio de Ciencia e Innovación AGL2008-05708-C02-02 Agència de Gestió d'Ajuts Universitaris i de Recerca 2011-BE-100125
Nota:	Little is known about the host factor in the response to PRRSV vaccination. For this purpose, piglets were immunized with a commercial PRRSV-live vaccine and classified as high responders (HR) or low responders (LR) as regards to the frequencies of virus-specific IFN-γ-secreting cells. Six weeks post vaccination, PBMCs isolated from three individuals with the most extreme responses in each HR and LR groups and 3 unvaccinated controls, were either stimulated with phytohaemagglutinin, challenged with the vaccine or mock treated for 24 h, prior conducting transcriptional studies, gene ontology and pathway analyses. The LR group had very low neutralizing antibody levels and showed a higher number of down-regulated transcripts compared with the HR group (FDR < 0.2, P < 0.001). Down-regulated genes encoded chemoattractants, proinflammatory cytokines and the interferon-inducible GBP family, and showed enrichment in wounding (FDR < 3.6E-13), inflammation (FDR < 8E-12), defence (FDR < 8.7E-09) and immunity (FDR < 7.6E08), suggesting immune response impairment. In the HR group, down-regulated genes were involved in protein transport (FDR < 4.77E-03), locomotory behavior (FDR < 5.47E-3), regulation of protein localization (FDR < 1.02E-02), and regulation of TNF superfamily member 15 and miR181. In contrast, the HR group presented up-regulated transcripts associated with wounding (FDR < 4.95). Moreover, IFN-γ was predicted to be an inhibited upstream regulator since IFN-γ pathways were associated with higher number of down-regulated genes in the LR (n = 40) than the HR (n = 10). Divergent responses to PRRSV-vaccination may be the result of the genetic background of the host.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Porcs
Publicat a:	Veterinary research, Vol. 47 (october 2016) , ISSN 1297-9716

DOI: 10.1186/s13567-016-0392-3
PMID: 27765052

12 p, 1.5 MB

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