Culicoides Midge Bites Modulate the Host Response and Impact on Bluetongue Virus Infection in Sheep
Pagès Martínez, Nonito (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Bréard, Emmanuel (Institut National de la Recherche Agronomique. Ecole Nationale Vétérinaire d'Alfort)
Urien, Céline (Institut National de la Recherche Agronomique. Virologie et Immunologie Moléculaires)
Talavera, Sandra (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Viarouge, Cyril (Institut National de la Recherche Agronomique. Ecole Nationale Vétérinaire d'Alfort)
Lorca Oró, Cristina (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Jouneau, Luc (Institut National de la Recherche Agronomique. Virologie et Immunologie Moléculaires)
Charley, Bernard (Institut National de la Recherche Agronomique. Virologie et Immunologie Moléculaires)
Zientara, Stéphan (Institut National de la Recherche Agronomique. Ecole Nationale Vétérinaire d'Alfort)
Bensaid, Albert (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Solanes, David (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Pujols, Joan (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Schwartz-Cornil, Isabelle (Institut National de la Recherche Agronomique. Virologie et Immunologie Moléculaires)

Date:	2014
Abstract:	Many haematophagous insects produce factors that help their blood meal and coincidently favor pathogen transmission. However nothing is known about the ability of Culicoides midges to interfere with the infectivity of the viruses they transmit. Among these, Bluetongue Virus (BTV) induces a hemorrhagic fever- type disease and its recent emergence in Europe had a major economical impact. We observed that needle inoculation of BTV8 in the site of uninfected C. nubeculosus feeding reduced viraemia and clinical disease intensity compared to plain needle inoculation. The sheep that developed the highest local inflammatory reaction had the lowest viral load, suggesting that the inflammatory response to midge bites may participate in the individual sensitivity to BTV viraemia development. Conversely compared to needle inoculation, inoculation of BTV8 by infected C. nubeculosus bites promoted viraemia and clinical symptom expression, in association with delayed IFN- induced gene expression and retarded neutralizing antibody responses. The effects of uninfected and infected midge bites on BTV viraemia and on the host response indicate that BTV transmission by infected midges is the most reliable experimental method to study the physio-pathological events relevant to a natural infection and to pertinent vaccine evaluation in the target species. It also leads the way to identify the promoting viral infectivity factors of infected Culicoides in order to possibly develop new control strategies against BTV and other Culicoides transmitted viruses.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Llengua blava
Published in:	PloS one, Vol. 9 (january 2014) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0083683
PMID: 24421899

9 p, 995.5 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles