Analysis of Serum miRNA Profiles of Myasthenia Gravis Patients
Nogales, Gisela (Institut d'Investigació Biomèdica Sant Pau)
Ramos-Fransi, Alba (Institut d'Investigació Biomèdica Sant Pau)
Suarez-Calvet, Xavier (Institut d'Investigació Biomèdica Sant Pau)
Navas Madroñal, Miquel (Institut d'Investigació Biomèdica Sant Pau)
Rojas-Garcia, Ricard (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Mosquera, José Luis (Universitat de Barcelona. Departament d'Estadística)
Diaz-Manera, Jordi (Institut d'Investigació Biomèdica Sant Pau)
Querol, Luis (Institut d'Investigació Biomèdica Sant Pau)
Gallardo, Eduard (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Illa, Isabel (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Universitat Autònoma de Barcelona

Date:	2014
Abstract:	Myasthenia gravis (MG) is an autoimmune disease characterized by the presence of autoantibodies, mainly against the acetylcholine receptor (AChR). The mechanisms triggering and maintaining this chronic disease are unknown. MiRNAs are regulatory molecules that play a key role in the immune system and are altered in many autoimmune diseases. The aim of this study was to evaluate miRNA profiles in serum of 61 AChR MG patients. We studied serum from patients with early onset MG (n = 22), late onset MG (n = 27) and thymoma (n = 12), to identify alterations in the specific subgroups. In a discovery cohort, we analysed 381 miRNA arrays from 5 patients from each subgroup, and 5 healthy controls. The 15 patients had not received any treatment. We found 32 miRNAs in different levels in MG and analysed 8 of these in a validation cohort that included 46 of the MG patients. MiR15b, miR122, miR-140-3p, miR185, miR192, miR20b and miR-885-5p were in lower levels in MG patients than in controls. Our study suggests that different clinical phenotypes in MG share common altered mechanisms in circulating miRNAs, with no additional contribution of the thymoma. MG treatment intervention does not modify the profile of these miRNAs. Novel insights into the pathogenesis of MG can be reached by the analysis of circulating miRNAs since some of these miRNAs have also been found low in MG peripheral mononuclear cells, and have targets with important roles in B cell survival and antibody production.
Grants:	Instituto de Salud Carlos III FIS 09/1964 Instituto de Salud Carlos III FIS CD10/00027 Instituto de Salud Carlos III FIS CM09/00017
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 9 (march 2014) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0091927
PMID: 24637658

11 p, 701.5 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles