HPV Testing by cobas HPV Test in a Population from Catalonia
Lloveras, Belén (Hospital del Mar (Barcelona, Catalunya))
Gomez, Silvia (Hospital del Mar (Barcelona, Catalunya))
Alameda, Francesc (Hospital del Mar (Barcelona, Catalunya))
Bellosillo Paricio, Beatriz (Hospital del Mar (Barcelona, Catalunya))
Mojal, Sergi (Institut Hospital del Mar d'Investigacions Mèdiques)
Muset, Mercè (Hospital del Mar (Barcelona, Catalunya))
Parra, Manuel (Hospital Universitario Virgen de Valme (Sevilla, Andalusia))
Palomares, José Carlos (Hospital Universitario Virgen de Valme (Sevilla, Andalusia))
Serrano, Sergi (Hospital del Mar (Barcelona, Catalunya))
Universitat Autònoma de Barcelona. Departament de Ciències Morfològiques

Date:	2013
Abstract:	HPV testing in cervical cancer screening has been proposed as an alternative or complementary to cytology in women older than 30 years. However, adequate clinical sensitivity and specificity are crucial for a new test to be implemented. Hybrid Capture 2 (HC2) has proved good clinical performance in selecting women at risk for high-grade intraepithelial lesions with a high sensitivity and specificity. cobas HPV Test has been recently launched and its performance in different clinical settings needs to be determined. The aim of this study was to evaluate the cobas HPV Test for the detection of cervical HPV infection in a population of women in Catalonia (Spain) using HC2 as a reference. Cervical liquid cytology samples from 958 women have been studied. Sensitivity was analyzed in 60 samples from patients with a high-grade intraepithelial lesion (≥CIN2) on histology and specificity was determined in 898 samples from women with no ≥CIN2. All cases had HC2 and cobas HPV Test performed. Statistical analyses of sensitivity, specificity and comparison between HC2 and cobas HPV Test by a non-inferiority test were applied. Sensitivity of HC2 and cobas HPV Test for detecting ≥CIN2 proved identical (98. 3%) while specificity was 85. 3% and 86. 2% respectively. The non-inferiority test demonstrated that cobas HPV Test surpassed 90% sensitivity and 98% specificity of HC2. The cobas HPV Test results fulfilled sensitivity and specificity requirements for HPV based cervical cancer screening and for the triage of minor cytological abnormalities, allowing its introduction in clinical settings.
Grants:	Ministerio de Ciencia e Innovación RD09/0076/00036
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 8 (march 2013) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0058153
PMID: 23483984

5 p, 139.2 KB

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