Cytochrome c Deficiency Differentially Affects the In Vivo Mitochondrial Electron Partitioning and Primary Metabolism Depending on the Photoperiod
Florez-Sarasa, Igor (Centre de Recerca en Agrigenòmica)
Welchen, Elina (Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral)
Racca, Sofia (Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral)
Gonzalez, Daniel H. (Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral)
Vallarino, José G. (Max-Planck-Institute of Molecular Plant Physiology)
Fernie, Alisdair (Max-Planck-Institute of Molecular Plant Physiology)
Ribas-Carbo, Miquel (Universitat de les Illes Balears)
Fernández Del-Saz, Néstor (Universidad de Concepción. Departamento de Botánica)

Date:	2021
Abstract:	Plant respiration provides metabolic flexibility under changing environmental conditions by modulating the activity of the nonphosphorylating alternative pathways from the mitochondrial electron transport chain, which bypass the main energy-producing components of the cytochrome oxidase pathway (COP). While adjustments in leaf primary metabolism induced by changes in day length are well studied, possible differences in the in vivo contribution of the COP and the alternative oxidase pathway (AOP) between different photoperiods remain unknown. In our study, in vivo electron partitioning between AOP and COP and expression analysis of respiratory components, photosynthesis, and the levels of primary metabolites were studied in leaves of wild-type (WT) plants and cytochrome c (CYTc) mutants, with reduced levels of COP components, under short- and long-day photoperiods. Our results clearly show that differences in AOP and COP in vivo activities between WT and cytc mutants depend on the photoperiod likely due to energy and stress signaling constraints. Parallel responses observed between in vivo respiratory activities, TCA cycle intermediates, amino acids, and stress signaling metabolites indicate the coordination of different pathways of primary metabolism to support growth adaptation under different photoperiods.
Grants:	European Commission 753301 Ministerio de Ciencia e Innovación RYC-2019-027244-I
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Alternative oxidase (AOX) ; Cytochrome c (CYTc) ; Oxygen isotope discrimination ; Metabolite profiling ; Photoperiod ; Primary metabolism
Published in:	Plants, Vol. 10, Issue 3 (March 2021) , art. 444, ISSN 2223-7747

DOI: 10.3390/plants10030444
PMID: 33652808

18 p, 1.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
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