Assessing the degradation of ancient milk proteins through site-specific deamidation patterns
Ramsøe, Abigail (Natural History Museum. Department of Earth Sciences)
Crispin, Mia (University of York. Department of Archaeology)
Mackie, Meaghan (University of Copenhagen. The Novo Nordisk Foundation Center for Protein Research)
McGrath, Krista Michelle (Universitat Autònoma de Barcelona. Institut de Ciència i Tecnologia Ambientals)
Fischer, Roman (University of Oxford. Target Discovery Institute, Nuffield Department of Medicine)
Demarchi, Beatrice (University of Turin. Department of Life Sciences and Systems Biology)
Collins, Matthew (University of Cambridge. McDonald Institute for Archaeological Research)
Hendy, Jessica (University of York. Department of Archaeology)
Speller, Camilla (University of British Columbia. Department of Anthropology)
Universitat Autònoma de Barcelona. Departament de Prehistòria

Data:	2021
Resum:	The origins, prevalence and nature of dairying have been long debated by archaeologists. Within the last decade, new advances in high-resolution mass spectrometry have allowed for the direct detection of milk proteins from archaeological remains, including ceramic residues, dental calculus, and preserved dairy products. Proteins recovered from archaeological remains are susceptible to post-excavation and laboratory contamination, a particular concern for ancient dairying studies as milk proteins such as beta-lactoglobulin (BLG) and caseins are potential laboratory contaminants. Here, we examine how site-specific rates of deamidation (i. e. , deamidation occurring in specific positions in the protein chain) can be used to elucidate patterns of peptide degradation, and authenticate ancient milk proteins. First, we characterize site-specific deamidation patterns in modern milk products and experimental samples, confirming that deamidation occurs primarily at low half-time sites. We then compare this to previously published palaeoproteomic data from six studies reporting ancient milk peptides. We confirm that site-specific deamidation rates, on average, are more advanced in BLG recovered from ancient dental calculus and pottery residues. Nevertheless, deamidation rates displayed a high degree of variability, making it challenging to authenticate samples with relatively few milk peptides. We demonstrate that site-specific deamidation is a useful tool for identifying modern contamination but highlight the need for multiple lines of evidence to authenticate ancient protein data.
Nota:	Unidad de excelencia María de Maeztu CEX2019-000940-M
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Proteins ; Proteome informatics ; Environmental social sciences
Publicat a:	Scientific reports, Vol. 11 (April 2021) , art. 7795, ISSN 2045-2322

DOI: 10.1038/s41598-021-87125-x
PMID: 33833277

14 p, 1.5 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > Institut de Ciència i Tecnologia Ambientals (ICTA)
Articles > Articles de recerca
Articles > Articles publicats