Metabolomics and transcriptomics to decipher molecular mechanisms underlying ectomycorrhizal root colonization of an oak tree
Sebastiana, M. (Universidade de Lisboa. Instituto de Biosistemas e Ciências Integrativas)
Gargallo-Garriga, Albert (Czech Academy of Sciences. Global Change Research Institute)
Sardans i Galobart, Jordi (Centre de Recerca Ecològica i d'Aplicacions Forestals)
Pérez-Trujillo, Míriam (Universitat Autònoma de Barcelona. Servei de Ressonància Magnètica Nuclear)
Monteiro, F. (Universidade de Lisboa. Instituto Superior de Agronomia)
Figueiredo, Andreia (Universidade de Lisboa. Instituto de Biosistemas e Ciências Integrativas)
Maia, M. (Universidade de Lisboa. Instituto de Biosistemas e Ciências Integrativas)
Nascimento, R. (Universidade de Lisboa. Instituto de Biosistemas e Ciências Integrativas)
Silva, M. Sousa (Universidade de Lisboa. Departamento de Química e Bioquímica)
Ferreira, A. N. (Universidade de Lisboa. Departamento de Química e Bioquímica)
Cordeiro, C. (Universidade de Lisboa. Departamento de Química e Bioquímica)
Marques, A. P. (Universidade de Lisboa. Departamento de Química e Bioquímica)
Sousa, L. (Universidade de Lisboa. Centro de Estatística e Aplicações)
Malhó, R. (Universidade de Lisboa. Instituto de Biosistemas e Ciências Integrativas)
Peñuelas, Josep (Centre de Recerca Ecològica i d'Aplicacions Forestals)

Data:	2021
Resum:	Mycorrhizas are known to have a positive impact on plant growth and ability to resist major biotic and abiotic stresses. However, the metabolic alterations underlying mycorrhizal symbiosis are still understudied. By using metabolomics and transcriptomics approaches, cork oak roots colonized by the ectomycorrhizal fungus Pisolithus tinctorius were compared with non-colonized roots. Results show that compounds putatively corresponding to carbohydrates, organic acids, tannins, long-chain fatty acids and monoacylglycerols, were depleted in ectomycorrhizal cork oak colonized roots. Conversely, non-proteogenic amino acids, such as gamma-aminobutyric acid (GABA), and several putative defense-related compounds, including oxylipin-family compounds, terpenoids and B6 vitamers were induced in mycorrhizal roots. Transcriptomic analysis suggests the involvement of GABA in ectomycorrhizal symbiosis through increased synthesis and inhibition of degradation in mycorrhizal roots. Results from this global metabolomics analysis suggest decreases in root metabolites which are common components of exudates, and in compounds related to root external protective layers which could facilitate plant-fungal contact and enhance symbiosis. Root metabolic pathways involved in defense against stress were induced in ectomycorrhizal roots that could be involved in a plant mechanism to avoid uncontrolled growth of the fungal symbiont in the root apoplast. Several of the identified symbiosis-specific metabolites, such as GABA, may help to understand how ectomycorrhizal fungi such as P. tinctorius benefit their host plants.
Ajuts:	European Commission 731077
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Molecular biology ; Plant sciences
Publicat a:	Scientific reports, Vol. 11 (April 2021) , art. 8576, ISSN 2045-2322

DOI: 10.1038/s41598-021-87886-5
PMID: 33883599

16 p, 2.6 MB

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