Purification and Characterization of a Novel Thermostable Papain Inhibitor from Moringa oleifera with Antimicrobial and Anticoagulant Properties
Cotabarren, Juliana (Universidad Nacional de La Plata. Departamento de Ciencias Biológicas)
Claver, Santiago (Universidad Nacional de La Plata. Departamento de Ciencias Biológicas)
Payrol, Juan Abreu (Escuela Latinoamericana de Medicina)
Garcia-Pardo, Javier (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Obregón, Walter David (Universidad Nacional de La Plata. Departamento de Ciencias Biológicas)
Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular

Date:	2021
Abstract:	Plant cystatins (or phytocystatins) comprise a large superfamily of natural bioactive small proteins that typically act as protein inhibitors of papain-like cysteine proteases. In this report, we present the purification and characterization of the first phytocystatin isolated from Moringa oleifera (MoPI). MoPI has a molecular mass of 19 kDa and showed an extraordinary physicochemical stability against acidic pHs and high temperatures. Our findings also revealed that MoPI is one of the most potent cysteine protease inhibitors reported to date, with K i and IC values of 2. 1 nM and 5. 7 nM, respectively. More interestingly, MoPI presents a strong antimicrobial activity against human pathogens such as Enterococcus faecalis and Staphylococcus aureus. In addition, MoPI also showed important anticoagulant activity, which is an unprecedented property for this family of protease inhibitors. These results highlight the pharmaceutical potential of this plant and its derived bioactive molecules.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Moringa oleifera ; Protease ; Plant protease inhibitor ; Phytocystatin ; Papain inhibitor ; Antibacterial ; Anticoagulant ; Bioactive compound
Published in:	Pharmaceutics, Vol. 13, Issue 4 (April 2021) , art. 512, ISSN 1999-4923

DOI: 10.3390/pharmaceutics13040512
PMID: 33917878

11 p, 827.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
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