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| Pàgina inicial > Articles > Articles publicats > Crohn's Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat |
(Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas) (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas) (Hospital Universitari Vall d'Hebron. Institut de Recerca) (Hospital Universitari Vall d'Hebron. Institut de Recerca) (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas) (Universitat Rovira i Virgili) (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)| Data: | 2021 |
| Resum: | Our understanding of the interplay between human adipose tissue and the immune system is limited. The mesothelium, an immunologically active structure, emerged as a source of visceral adipose tissue. After investigating the mesothelial properties of human visceral and subcutaneous adipose tissue and their progenitors, we explored whether the dysfunctional obese and Crohn's disease environments influence the mesothelial/mesenchymal properties of their adipocyte precursors, as well as their ability to mount an immune response. Using a tandem transcriptomic/proteomic approach, we evaluated the mesothelial and mesenchymal expression profiles in adipose tissue, both in subjects covering a wide range of body-mass indexes and in Crohn's disease patients. We also isolated adipose tissue precursors (adipose-derived stem cells, ASCs) to assess their mesothelial/mesenchymal properties, as well as their antigen-presenting features. Human visceral tissue presented a mesothelial phenotype not detected in the subcutaneous fat. Only ASCs from mesenteric adipose tissue, named creeping fat, had a significantly higher expression of the hallmark mesothelial genes mesothelin (MSLN) and Wilms' tumor suppressor gene 1 (WT1), supporting a mesothelial nature of these cells. Both lean and Crohn's disease visceral ASCs expressed equivalent surface percentages of the antigen-presenting molecules human leucocyte antigen-DR isotype (HLA-DR) and CD86. However, lean-derived ASCs were predominantly HLA-DR dim, whereas in Crohn's disease, the HLA-DR bright subpopulation was increased 3. 2-fold. Importantly, the mesothelial-enriched Crohn's disease precursors activated CD4 + T-lymphocytes. Our study evidences a mesothelial signature in the creeping fat of Crohn's disease patients and its progenitor cells, the latter being able to present antigens and orchestrate an immune response. |
| Ajuts: | Ministerio de Ciencia e Innovación PI14/00228 Ministerio de Ciencia e Innovación PI17/01503 Ministerio de Ciencia e Innovación SAF2015-65019-R Ministerio de Ciencia e Innovación BFU2015-70454-REDT Ministerio de Ciencia e Innovación BFU2017-90578-REDT Ministerio de Ciencia e Innovación BFU2016-76711-R Ministerio de Ciencia e Innovación PI15/00143 Ministerio de Ciencia e Innovación PI18/00037 Ministerio de Economía y Competitividad CB07708/0012 Ministerio de Economía y Competitividad CP10/00438 Ministerio de Economía y Competitividad CPII16/00008 |
| Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Llengua: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Matèria: | Crohn's disease ; Adipose tissue ; Mesothelium ; Adipose-derived stem cells |
| Publicat a: | International journal of molecular sciences, Vol. 22 (april 2021) , ISSN 1422-0067 |
