Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
Ancín, María (Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación)
Larraya, Luis (Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación)
Florez-Sarasa, Igor (Centre de Recerca en Agrigenòmica)
Bénard, Camille (Bordeaux University. Biologie du Fruit et Pathologie and Plateforme Metabolome Bordeaux)
Fernández-San Millán, Alicia (Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación)
Veramendi, Jon (Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación)
Gibon, Yves (Bordeaux University. Biologie du Fruit et Pathologie and Plateforme Metabolome Bordeaux)
Fernie, Alisdair (Max-Planck-Institut für Molekulare Pflanzenphysiologie)
Aranjuelo, Iker (Gobierno de Navarra. Instituto de Agrobiotecnología)
Farran, Inmaculada (Universidad Pública de Navarra. Departamento de Agronomía, Biotecnología y Alimentación)

Date:	2021
Abstract:	In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.
Grants:	Ministerio de Ciencia e Innovación AGL2016-79868 Ministerio de Educación, Cultura y Deporte FPU13/01675 European Commission 753301 Agencia Estatal de Investigación RYC2019-027244-I
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Carbon metabolism ; Chloroplast ; Glutamine synthetase ; GS-GOGAT pathway ; Nitrogen metabolism ; Photorespiration ; Thioredoxin
Published in:	Journal of experimental botany, Vol. 72, Issue 13 (May 2021) , p. 4949-4964, ISSN 1460-2431

DOI: 10.1093/jxb/erab193
PMID: 33963398

16 p, 1.8 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles