A replicating viral vector greatly enhances accumulation of helical virus-like particles in plants
Thuenemann, Eva (Norwich Research Park. John Innes Centre (UK))
Byrne, Matthew J. (University of Leeds. School of Molecular and Cellular Biology)
Peyret, Hadrien (Norwich Research Park. John Innes Centre (UK))
Saunders, Keith (Norwich Research Park. John Innes Centre (UK))
Castells-Graells, Roger (University of California. Department of Chemistry and Biochemistry)
Ferriol, Inmaculada (Consejo Superior de Investigaciones Científicas)
Santoni, Mattia (Diamante srl (Italy))
Steele, John F. C. (Norwich Research Park. John Innes Centre (UK))
Ranson, Neil (University of Leeds. School of Molecular and Cellular Biology)
Avesani, Linda (University of Verona. Department of Biotechnology)
Lopez-Moya, Juan Jose (Centre de Recerca en Agrigenòmica)
Lomonossoff, George (Norwich Research Park. John Innes Centre (UK))

Data:	2021
Resum:	The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X-based vector (pEff) and a non-replicating vector (pEAQ-HT). Significantly greater quantities of VLPs could be purified when pEff was used. The pEff system was also very efficient at producing VLPs of helical viruses from different virus families. Examination of the RNA content of AltMV and tobacco mosaic virus VLPs produced from pEff revealed the presence of vector-derived RNA sequences, suggesting that the replicating RNA acts as a scaffold for VLP assembly. Cryo-EM analysis of the AltMV VLPs showed they had a structure very similar to that of authentic potexvirus particles. Thus, we conclude that vectors generating replicating forms of RNA, such as pEff, are very efficient for producing helical VLPs.
Ajuts:	European Commission 774078 Agencia Estatal de Investigación PID2019-105692RB-I00 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Ciencia e Innovación CEX2019-000902-S
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya; JIC-CRAG secondment program (CA759C05B)
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Virus-like particle ; Overexpression ; Filamentous ; Rod-shaped ; Peaq-ht ; Peff ; Potexvirus ; Potyviridae ; High-resolution cryo-EM
Publicat a:	Viruses, Vol. 13, Núm. 5 (May 2021) , art. 885, ISSN 1999-4915

DOI: 10.3390/v13050885
PMID: 34064959

13 p, 1.8 MB

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