Purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
Cotabarren, Juliana (Universidad Nacional de La Plata. Departamento de Ciencias Biológicas)
Ozón, Brenda (Universidad Nacional de La Plata. Departamento de Ciencias Biológicas)
Claver, Santiago (Universidad Nacional de La Plata. Departamento de Ciencias Biológicas)
Garcia-Pardo, Javier (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Obregón, Walter David (Universidad Nacional de La Plata. Departamento de Ciencias Biológicas)
Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular

Date:	2021
Abstract:	Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from Geoffroea decorticans seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC) of 35. 5 ± 0. 3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257. 199 Da, 2717. 165 Da, and 5422. 002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of Arachis hypogaea. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Geoffroea decorticans ; Antioxidant ; Functional compound ; Thermostable protein ; Reactive oxygen species ; Anticoagulant ; Bioactive compound
Published in:	Pharmaceutics, Vol. 13, Issue 8 (August 2021) , art. 1153, ISSN 1999-4923

DOI: 10.3390/pharmaceutics13081153
PMID: 34452114

12 p, 1.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
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